Detailed information of TA system TA050693

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 13570..13839
• Replicon: plasmid A
• Accession: NZ_CP010138
• Organism: Escherichia coli strain D2

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: RG35_RS25055
• Protein ID: WP_001312861.1
• Coordinates: 13681..13839 (+)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	13570..13635 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
RG35_RS25025	9363..9890	+	528	WP_000290793.1	single-stranded DNA-binding protein	-
RG35_RS25030	9946..10179	+	234	WP_000006004.1	DUF905 domain-containing protein	-
RG35_RS25035	10238..12196	+	1959	WP_001145469.1	ParB/RepB/Spo0J family partition protein	-
RG35_RS25040	12251..12685	+	435	WP_000845901.1	conjugation system SOS inhibitor PsiB	-
RG35_RS25045	12682..13401	+	720	WP_001276234.1	plasmid SOS inhibition protein A	-
RG35_RS27090	13413..13601	-	189	WP_001299721.1	hypothetical protein	-
-	13413..13637	+	225	NuclAT_0	-	-
-	13413..13637	+	225	NuclAT_0	-	-
-	13413..13637	+	225	NuclAT_0	-	-
-	13413..13637	+	225	NuclAT_0	-	-
-	13570..13635	+	66	-	-	Antitoxin
RG35_RS25055	13681..13839	+	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
RG35_RS25075	14758..15045	+	288	WP_000107537.1	hypothetical protein	-
RG35_RS25080	15166..15987	+	822	WP_001234469.1	DUF945 domain-containing protein	-
RG35_RS25085	16284..16931	-	648	WP_000614282.1	transglycosylase SLT domain-containing protein	-
RG35_RS25095	17217..17600	+	384	WP_001151564.1	relaxosome protein TraM	-
RG35_RS25100	17794..18480	+	687	WP_000332487.1	PAS domain-containing protein	-
RG35_RS25105	18574..18801	+	228	WP_001254388.1	conjugal transfer relaxosome protein TraY	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaTEM-1B / tet(A) / aph(6)-Id / aph(3'')-Ib / sul2 / mph(A) / sul1 / qacE / aadA5 / aac(3)-IId	-	1..83922	83922

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T50693 WP_001312861.1 NZ_CP010138:13681-13839 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T50693 NZ_CP010138:13681-13839 [Escherichia coli]
ATGAAACTACCACGAAGTTCCCTTGTCTGGTGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGACACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 66 bp

>AT50693 NZ_CP010138:13570-13635 [Escherichia coli]
AGAAGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCAC

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin