Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 3878474..3879015 Replicon chromosome
Accession NZ_CP010137
Organism Escherichia coli strain D2

Toxin (Protein)


Gene name yafQ Uniprot ID A0A140NAP5
Locus tag RG35_RS20415 Protein ID WP_000615981.1
Coordinates 3878737..3879015 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID S1EWQ0
Locus tag RG35_RS20410 Protein ID WP_000729704.1
Coordinates 3878474..3878734 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
RG35_RS20375 3874157..3874942 - 786 WP_000207564.1 putative lateral flagellar export/assembly protein LafU -
RG35_RS20380 3874914..3876626 + 1713 Protein_3665 flagellar biosynthesis protein FlhA -
RG35_RS20395 3876842..3877339 - 498 WP_000006260.1 REP-associated tyrosine transposase RayT -
RG35_RS20405 3877515..3878288 - 774 WP_162780054.1 C40 family peptidase -
RG35_RS20410 3878474..3878734 + 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
RG35_RS20415 3878737..3879015 + 279 WP_000615981.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
RG35_RS20420 3879171..3879911 + 741 WP_001225679.1 murein L,D-transpeptidase -
RG35_RS20425 3879882..3880649 - 768 WP_000333379.1 class II glutamine amidotransferase -
RG35_RS20430 3880855..3881433 - 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(6-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10860.65 Da        Isoelectric Point: 10.0702

>T50688 WP_000615981.1 NZ_CP010137:3878737-3879015 [Escherichia coli]
MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNTLPLPAVYKDHPLQSSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T50688 NZ_CP010137:3878737-3879015 [Escherichia coli]
ATGATTCAAAGGGATATTGAATACTCGGGACAATTTTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATACTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAA
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTATTTGGGTAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9391.77 Da        Isoelectric Point: 5.0758

>AT50688 WP_000729704.1 NZ_CP010137:3878474-3878734 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT50688 NZ_CP010137:3878474-3878734 [Escherichia coli]
ATGGCTGCTAACGCGTTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCGGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAAGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATTAAAAACAGCGAAGCTGGCGTTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4MMG
PDB 4MMJ
PDB 4ML2
PDB 4ML0

Antitoxin

Source ID Structure
PDB 4ML0
AlphaFold DB A0A0E0Y6Z6

References