Detailed information of TA system TA050687

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 3872285..3872979
• Replicon: chromosome
• Accession: NZ_CP010137
• Organism: Escherichia coli strain D2

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: S1EXB8
• Locus tag: RG35_RS20360
• Protein ID: WP_001263493.1
• Coordinates: 3872285..3872683 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1FJN6
• Locus tag: RG35_RS20365
• Protein ID: WP_000554757.1
• Coordinates: 3872686..3872979 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
RG35_RS20330	3867285..3868529	-	1245	WP_000189541.1	esterase FrsA	-
-	3867945..3868025	-	81	NuclAT_11	-	-
-	3867945..3868025	-	81	NuclAT_11	-	-
-	3867945..3868025	-	81	NuclAT_11	-	-
-	3867945..3868025	-	81	NuclAT_11	-	-
RG35_RS20335	3868621..3869079	-	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
RG35_RS20340	3869340..3870797	+	1458	WP_001293003.1	cytosol nonspecific dipeptidase	-
RG35_RS20345	3870854..3871375	-	522	Protein_3658	peptide chain release factor H	-
RG35_RS20350	3871374..3871577	-	204	Protein_3659	RNA ligase RtcB family protein	-
RG35_RS20355	3871823..3872275	-	453	WP_001059847.1	GNAT family N-acetyltransferase	-
RG35_RS20360	3872285..3872683	-	399	WP_001263493.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
RG35_RS20365	3872686..3872979	-	294	WP_000554757.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
RG35_RS20370	3873031..3874086	-	1056	WP_001226164.1	DNA polymerase IV	-
RG35_RS20375	3874157..3874942	-	786	WP_000207564.1	putative lateral flagellar export/assembly protein LafU	-
RG35_RS20380	3874914..3876626	+	1713	Protein_3665	flagellar biosynthesis protein FlhA	-
RG35_RS20395	3876842..3877339	-	498	WP_000006260.1	REP-associated tyrosine transposase RayT	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15472.83 Da
• Isoelectric Point: 8.0949

>T50687 WP_001263493.1 NZ_CP010137:c3872683-3872285 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKQARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T50687 NZ_CP010137:c3872683-3872285 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGTGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACAGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.81 Da
• Isoelectric Point: 5.9050

>AT50687 WP_000554757.1 NZ_CP010137:c3872979-3872686 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT50687 NZ_CP010137:c3872979-3872686 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATTACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGAGCT
GAAAAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Structures

Toxin

• AlphaFold DB: S1EXB8

Antitoxin

• AlphaFold DB: S1FJN6