Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 4949114..4949616 Replicon chromosome
Accession NZ_CP010134
Organism Escherichia coli strain D1

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag RG34_RS25975 Protein ID WP_000767829.1
Coordinates 4949362..4949616 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag RG34_RS25970 Protein ID WP_001259255.1
Coordinates 4949114..4949365 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
RG34_RS25940 4944292..4945359 - 1068 WP_000080085.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -
RG34_RS25945 4945359..4946429 - 1071 WP_000108980.1 histidinol-phosphate transaminase -
RG34_RS25950 4946426..4947730 - 1305 WP_001298841.1 histidinol dehydrogenase -
RG34_RS25955 4947736..4948635 - 900 WP_000131782.1 ATP phosphoribosyltransferase -
RG34_RS25965 4948781..4948831 - 51 WP_001368802.1 his operon leader peptide -
RG34_RS25970 4949114..4949365 + 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
RG34_RS25975 4949362..4949616 + 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
RG34_RS25980 4949699..4950523 + 825 WP_000754737.1 SDR family oxidoreductase -
RG34_RS25985 4950569..4951498 + 930 WP_000803351.1 LysR family transcriptional regulator -
RG34_RS28915 4951713..4951775 + 63 WP_010723108.1 membrane protein YoeI -
RG34_RS25990 4951765..4953123 + 1359 WP_000019197.1 putrescine/proton symporter PlaP -
RG34_RS25995 4953302..4954360 + 1059 WP_000492346.1 transport protein YeeE -
RG34_RS26000 4954374..4954601 + 228 WP_000985280.1 sulfurtransferase TusA family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T50665 WP_000767829.1 NZ_CP010134:4949362-4949616 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T50665 NZ_CP010134:4949362-4949616 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT50665 WP_001259255.1 NZ_CP010134:4949114-4949365 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT50665 NZ_CP010134:4949114-4949365 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References