Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 3875554..3876056 Replicon chromosome
Accession NZ_CP010132
Organism Escherichia coli strain C10

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag RG32_RS20265 Protein ID WP_000767829.1
Coordinates 3875554..3875808 (-) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag RG32_RS20270 Protein ID WP_001259255.1
Coordinates 3875805..3876056 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
RG32_RS20240 3870569..3870796 - 228 WP_000985273.1 sulfurtransferase TusA family protein -
RG32_RS20245 3870810..3871868 - 1059 WP_000492346.1 transport protein YeeE -
RG32_RS20250 3872047..3873405 - 1359 WP_000019197.1 putrescine/proton symporter PlaP -
RG32_RS26890 3873395..3873457 - 63 WP_010723108.1 membrane protein YoeI -
RG32_RS20255 3873672..3874601 - 930 WP_000803351.1 LysR family transcriptional regulator -
RG32_RS20260 3874647..3875471 - 825 WP_000754758.1 SDR family oxidoreductase -
RG32_RS20265 3875554..3875808 - 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
RG32_RS20270 3875805..3876056 - 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
RG32_RS20275 3876339..3876389 + 51 WP_001364200.1 his operon leader peptide -
RG32_RS20285 3876535..3877434 + 900 WP_000131782.1 ATP phosphoribosyltransferase -
RG32_RS20290 3877440..3878744 + 1305 WP_001542955.1 histidinol dehydrogenase -
RG32_RS20295 3878741..3879811 + 1071 WP_000108941.1 histidinol-phosphate transaminase -
RG32_RS20300 3879811..3880878 + 1068 WP_000080102.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T50614 WP_000767829.1 NZ_CP010132:c3875808-3875554 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T50614 NZ_CP010132:c3875808-3875554 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGACGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT50614 WP_001259255.1 NZ_CP010132:c3876056-3875805 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT50614 NZ_CP010132:c3876056-3875805 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References