Detailed information of TA system TA050592

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 1055406..1056008
• Replicon: chromosome
• Accession: NZ_CP010132
• Organism: Escherichia coli strain C10

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: RG32_RS05520
• Protein ID: WP_000897305.1
• Coordinates: 1055406..1055717 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: RG32_RS05525
• Protein ID: WP_000356397.1
• Coordinates: 1055718..1056008 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
RG32_RS05490	1050436..1051221	+	786	WP_000059678.1	DeoR/GlpR family DNA-binding transcription regulator	-
RG32_RS05495	1051320..1051919	+	600	WP_001295269.1	glucose-1-phosphatase	-
RG32_RS05500	1051913..1052785	+	873	WP_000920762.1	virulence factor BrkB family protein	-
RG32_RS05505	1052782..1053219	+	438	WP_000560983.1	D-tyrosyl-tRNA(Tyr) deacylase	-
RG32_RS05510	1053264..1054205	+	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
RG32_RS05515	1054269..1055177	-	909	WP_001299484.1	alpha/beta hydrolase	-
RG32_RS05520	1055406..1055717	+	312	WP_000897305.1	hypothetical protein	Toxin
RG32_RS05525	1055718..1056008	+	291	WP_000356397.1	helix-turn-helix domain-containing protein	Antitoxin
RG32_RS05535	1056594..1056812	+	219	WP_001295676.1	ribbon-helix-helix domain-containing protein	-
RG32_RS05540	1057031..1057273	+	243	WP_001086388.1	hypothetical protein	-
RG32_RS05550	1057502..1057873	-	372	Protein_996	IS110 family transposase	-
RG32_RS05560	1057946..1059123	+	1178	WP_198646305.1	IS3 family transposase	-
RG32_RS05565	1059311..1060240	-	930	WP_000027708.1	formate dehydrogenase accessory protein FdhE	-
RG32_RS05570	1060237..1060872	-	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T50592 WP_000897305.1 NZ_CP010132:1055406-1055717 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T50592 NZ_CP010132:1055406-1055717 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTTTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT50592 WP_000356397.1 NZ_CP010132:1055718-1056008 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT50592 NZ_CP010132:1055718-1056008 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
GCGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCTGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7