Detailed information of TA system TA050584

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 60137..60401
• Replicon: plasmid A
• Accession: NZ_CP010130
• Organism: Escherichia coli strain C9

Toxin (Protein)

• Gene name: pndA
• Uniprot ID: E6BRV3
• Locus tag: RG31_RS24670
• Protein ID: WP_001303307.1
• Coordinates: 60137..60289 (-)
• Length: 51 a.a.

Antitoxin (RNA)

Gene name	pndB
Locus tag	-
Coordinates	60339..60401 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
RG31_RS24620	56331..56945	+	615	WP_000578649.1	plasmid IncI1-type surface exclusion protein ExcA	-
RG31_RS24625	57043..57252	+	210	WP_001140545.1	hemolysin expression modulator Hha	-
RG31_RS26605	57461..57637	+	177	WP_001054900.1	hypothetical protein	-
-	59376..59427	+	52	NuclAT_2	-	-
-	59376..59427	+	52	NuclAT_2	-	-
-	59376..59427	+	52	NuclAT_2	-	-
-	59376..59427	+	52	NuclAT_2	-	-
RG31_RS24665	59814..60065	+	252	WP_001291968.1	hypothetical protein	-
RG31_RS24670	60137..60289	-	153	WP_001303307.1	Hok/Gef family protein	Toxin
-	60339..60401	+	63	NuclAT_0	-	Antitoxin
-	60339..60401	+	63	NuclAT_0	-	Antitoxin
-	60339..60401	+	63	NuclAT_0	-	Antitoxin
-	60339..60401	+	63	NuclAT_0	-	Antitoxin
RG31_RS24675	60604..61695	+	1092	WP_000426061.1	hypothetical protein	-
-	61762..61822	+	61	NuclAT_1	-	-
-	61762..61822	+	61	NuclAT_1	-	-
-	61762..61822	+	61	NuclAT_1	-	-
-	61762..61822	+	61	NuclAT_1	-	-
RG31_RS24680	62002..63210	+	1209	WP_000121273.1	IncI1-type conjugal transfer protein TrbA	-
RG31_RS24685	63229..64299	+	1071	WP_000151582.1	IncI1-type conjugal transfer protein TrbB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..93062	93062

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (7-46)

Sequences

Toxin

Protein

• Length: 51 a.a.
• Molecular weight: 5817.21 Da
• Isoelectric Point: 8.7948

>T50584 WP_001303307.1 NZ_CP010130:c60289-60137 [Escherichia coli]
MPQRTFLMMLIVICVTILCFVWMVRDSLCVLRLQQGNTVLVATLAYEVKR

Nucleotide

• Length: 153 bp

>T50584 NZ_CP010130:c60289-60137 [Escherichia coli]
ATGCCACAGCGAACGTTTTTAATGATGTTAATCGTCATCTGTGTGACGATTCTGTGTTTTGTCTGGATGGTGAGGGATTC
GCTTTGCGTACTCCGGCTCCAGCAGGGAAACACAGTGCTTGTGGCAACGTTAGCCTACGAAGTTAAACGTTAA

Antitoxin

Nucleotide

• Length: 63 bp

>AT50584 NZ_CP010130:60339-60401 [Escherichia coli]
TCGAAGCATTACAGTAGAGCCCTTGGTTGATTGATAAAAAAATCTCCAGGGGCTTTCTGCTGT

Structures

Toxin

• AlphaFold DB: A0A634Q5L1

Antitoxin