Detailed information of TA system TA050579

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 3727957..3728651
• Replicon: chromosome
• Accession: NZ_CP010129
• Organism: Escherichia coli strain C9

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: S1EXB8
• Locus tag: RG31_RS20075
• Protein ID: WP_001263493.1
• Coordinates: 3727957..3728355 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1FJN6
• Locus tag: RG31_RS20080
• Protein ID: WP_000554757.1
• Coordinates: 3728358..3728651 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
RG31_RS20045	3722957..3724201	-	1245	WP_000189541.1	esterase FrsA	-
-	3723617..3723697	-	81	NuclAT_11	-	-
-	3723617..3723697	-	81	NuclAT_11	-	-
-	3723617..3723697	-	81	NuclAT_11	-	-
-	3723617..3723697	-	81	NuclAT_11	-	-
RG31_RS20050	3724293..3724751	-	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
RG31_RS20055	3725012..3726469	+	1458	WP_001293003.1	cytosol nonspecific dipeptidase	-
RG31_RS20060	3726526..3727047	-	522	Protein_3537	peptide chain release factor H	-
RG31_RS20065	3727046..3727249	-	204	Protein_3538	RNA ligase RtcB family protein	-
RG31_RS20070	3727495..3727947	-	453	WP_001059847.1	GNAT family N-acetyltransferase	-
RG31_RS20075	3727957..3728355	-	399	WP_001263493.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
RG31_RS20080	3728358..3728651	-	294	WP_000554757.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
RG31_RS20085	3728703..3729758	-	1056	WP_001226164.1	DNA polymerase IV	-
RG31_RS20090	3729829..3730614	-	786	WP_000207549.1	putative lateral flagellar export/assembly protein LafU	-
RG31_RS20095	3730586..3732298	+	1713	Protein_3544	flagellar biosynthesis protein FlhA	-
RG31_RS20110	3732514..3733011	-	498	WP_000006261.1	REP-associated tyrosine transposase RayT	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	gmhA/lpcA	3672167..3757924	85757

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15472.83 Da
• Isoelectric Point: 8.0949

>T50579 WP_001263493.1 NZ_CP010129:c3728355-3727957 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKQARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T50579 NZ_CP010129:c3728355-3727957 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGTGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACAGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.81 Da
• Isoelectric Point: 5.9050

>AT50579 WP_000554757.1 NZ_CP010129:c3728651-3728358 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT50579 NZ_CP010129:c3728651-3728358 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATTACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGAGCT
GAAAAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Structures

Toxin

• AlphaFold DB: S1EXB8

Antitoxin

• AlphaFold DB: S1FJN6