Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 1891705..1892207 Replicon chromosome
Accession NZ_CP010129
Organism Escherichia coli strain C9

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag RG31_RS09910 Protein ID WP_000767829.1
Coordinates 1891953..1892207 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag RG31_RS09905 Protein ID WP_001259255.1
Coordinates 1891705..1891956 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
RG31_RS09875 1886883..1887950 - 1068 WP_073510789.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -
RG31_RS09880 1887950..1889020 - 1071 WP_000108970.1 histidinol-phosphate transaminase -
RG31_RS09885 1889017..1890321 - 1305 WP_073510791.1 histidinol dehydrogenase -
RG31_RS09890 1890327..1891226 - 900 WP_046880201.1 ATP phosphoribosyltransferase -
RG31_RS09900 1891372..1891422 - 51 WP_001364200.1 his operon leader peptide -
RG31_RS09905 1891705..1891956 + 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
RG31_RS09910 1891953..1892207 + 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
RG31_RS09915 1892290..1893114 + 825 WP_000754749.1 SDR family oxidoreductase -
RG31_RS09920 1893160..1894089 + 930 WP_000803351.1 LysR family transcriptional regulator -
RG31_RS26170 1894304..1894366 + 63 WP_010723108.1 membrane protein YoeI -
RG31_RS09925 1894356..1895714 + 1359 WP_000019197.1 putrescine/proton symporter PlaP -
RG31_RS09930 1895893..1896951 + 1059 WP_073510793.1 transport protein YeeE -
RG31_RS09935 1896965..1897192 + 228 WP_000985280.1 sulfurtransferase TusA family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T50566 WP_000767829.1 NZ_CP010129:1891953-1892207 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T50566 NZ_CP010129:1891953-1892207 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACACCATTTGAAGGTAAGGGGAAACCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT50566 WP_001259255.1 NZ_CP010129:1891705-1891956 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT50566 NZ_CP010129:1891705-1891956 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References