Detailed information of TA system TA050554

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 85584..86001
• Replicon: plasmid A
• Accession: NZ_CP010126
• Organism: Escherichia coli strain C8

Toxin (Protein)

• Gene name: hok
• Uniprot ID: B1VC78
• Locus tag: RG30_RS25880
• Protein ID: WP_001302184.1
• Coordinates: 85843..86001 (+)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	85584..85778 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
RG30_RS27755	81288..81494	+	207	WP_000275848.1	hypothetical protein	-
RG30_RS25850	81520..82047	+	528	WP_073534308.1	single-stranded DNA-binding protein	-
RG30_RS25855	82113..82337	+	225	WP_073534359.1	DUF905 family protein	-
RG30_RS25860	82403..84367	+	1965	WP_001587501.1	ParB/RepB/Spo0J family partition protein	-
RG30_RS25865	84422..84856	+	435	WP_073534309.1	conjugation system SOS inhibitor PsiB	-
RG30_RS25870	84853..85572	+	720	WP_029400523.1	plasmid SOS inhibition protein A	-
-	85584..85778	+	195	NuclAT_0	-	Antitoxin
-	85584..85778	+	195	NuclAT_0	-	Antitoxin
-	85584..85778	+	195	NuclAT_0	-	Antitoxin
-	85584..85778	+	195	NuclAT_0	-	Antitoxin
RG30_RS25880	85843..86001	+	159	WP_001302184.1	type I toxin-antitoxin system Hok family toxin	Toxin
RG30_RS25890	86548..86833	+	286	Protein_91	hypothetical protein	-
RG30_RS27760	86953..87773	+	821	Protein_92	DUF945 domain-containing protein	-
RG30_RS25900	88069..88716	-	648	WP_149026021.1	transglycosylase SLT domain-containing protein	-
RG30_RS25915	88992..89375	+	384	WP_073534310.1	relaxosome protein TraM	-
RG30_RS25920	89570..90217	+	648	WP_073534311.1	transcriptional regulator TraJ family protein	-
RG30_RS25925	90336..90563	+	228	WP_033800806.1	conjugal transfer relaxosome protein TraY	-
RG30_RS25930	90597..90956	+	360	WP_073534312.1	type IV conjugative transfer system pilin TraA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	vat / paa / papH / papC / papD	1..143617	143617

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6108.35 Da
• Isoelectric Point: 9.1977

>T50554 WP_001302184.1 NZ_CP010126:85843-86001 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGYREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T50554 NZ_CP010126:85843-86001 [Escherichia coli]
ATGAAACTACCACGCAGCTCTCTTGTCTGGTGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGATACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 195 bp

>AT50554 NZ_CP010126:85584-85778 [Escherichia coli]
TCACACGGATTACCCGTAGCCTGAATGAGCGGGTTATTTTCAGGAAAAGTGAGTGTGGTCAGCGTGCAGGTATATGGGCT
ATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTGCCGTGTTGTGTGGCAGAAGGACAAA
AGCCCCGTAGTTAATTTTTCATTAACCCACGAGGC

Structures

Antitoxin