Detailed information of TA system TA050546

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 3462477..3463204
• Replicon: chromosome
• Accession: NZ_CP010125
• Organism: Escherichia coli strain C8

Toxin (Protein)

• Gene name: higB
• Uniprot ID: J7Q991
• Locus tag: RG30_RS17870
• Protein ID: WP_000547564.1
• Coordinates: 3462893..3463204 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: RG30_RS17865
• Protein ID: WP_000126291.1
• Coordinates: 3462477..3462896 (-)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
RG30_RS17845	3457526..3458053	-	528	WP_001078777.1	electron transport protein HydN	-
RG30_RS17850	3458202..3459212	-	1011	WP_001392554.1	DNA-binding transcriptional regulator AscG	-
RG30_RS17855	3459472..3460929	+	1458	WP_048963557.1	PTS cellobiose/arbutin/salicin transporter subunit IIBC	-
RG30_RS17860	3460938..3462362	+	1425	WP_000110323.1	6-phospho-beta-glucosidase AscB	-
RG30_RS17865	3462477..3462896	-	420	WP_000126291.1	helix-turn-helix domain-containing protein	Antitoxin
RG30_RS17870	3462893..3463204	-	312	WP_000547564.1	type II toxin-antitoxin system HigB family toxin	Toxin
RG30_RS17875	3463378..3464139	-	762	WP_073533968.1	hypothetical protein	-
RG30_RS17880	3464164..3464634	-	471	WP_000132961.1	hydrogenase maturation peptidase HycI	-
RG30_RS17885	3464627..3465037	-	411	WP_073533969.1	formate hydrogenlyase assembly protein HycH	-
RG30_RS17890	3465034..3465801	-	768	WP_000067399.1	formate hydrogenlyase subunit HycG	-
RG30_RS17895	3465801..3466343	-	543	WP_000493792.1	formate hydrogenlyase subunit HycF	-
RG30_RS17900	3466353..3468062	-	1710	WP_001288134.1	formate hydrogenlyase subunit HycE	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12426.13 Da
• Isoelectric Point: 9.7248

>T50546 WP_000547564.1 NZ_CP010125:c3463204-3462893 [Escherichia coli]
MHIISKAPFEESARKYPNDALALQALYRVIKETDFSTPEEMRTAFPNLDNFRYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T50546 NZ_CP010125:c3463204-3462893 [Escherichia coli]
ATGCACATCATTTCTAAAGCGCCATTTGAAGAGTCTGCGCGTAAATATCCTAACGACGCTCTGGCTCTTCAGGCATTGTA
TCGGGTTATTAAGGAAACGGATTTTTCTACACCAGAAGAAATGCGAACGGCGTTTCCCAACCTCGACAATTTTAGATATC
GCAACAAATGGTACGTTCTGGATGTCGGCGGGAACAATCTTAGGGTTATTGCCTACATAAACTTTGTTAATAAGCGGTTC
TTTGTGAAGCACATAACGAATCACGCAGAGTATGACAAACTGACACGTTACTACAGAGAGAACAAAGAATGA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15439.46 Da
• Isoelectric Point: 4.5628

>AT50546 WP_000126291.1 NZ_CP010125:c3462896-3462477 [Escherichia coli]
MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLASRIAEYENNHEKFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT50546 NZ_CP010125:c3462896-3462477 [Escherichia coli]
ATGACTGCTAATGCTGCACGCGCTGTTAAAGCAACAAGAGAACTGGTCAATGCAGTCCCCTTTCTGGGAGGTAGTGACTC
AGAAGATGATTATCGTGAAGCACTGGAACTGGTCGAATATTTGATTGAAGAAGATGACACCAATCCACTTATCGACTTTC
TGGCGAGCCGCATTGCAGAATATGAAAACAACCACGAAAAGTTTGCAGAGTTCGACAAAGCCGTTGCGGCAATGCCGGTT
GGCGTGGCGTTACTTCGTACGCTGATTGATCAACATAATCTGACCTACGCGGATTTGAAGAACGAAATCGGCTCTAAATC
CCTGGTCAGTCAGATTTTGTCTGGGCAACGATCCCTGACGATATCCCACATTAAGGCACTTTCAGCCCGATTTGGTGTTA
AGCCGGAATGGTTTCTTTGA

Structures

Toxin

• AlphaFold DB: A0A0A0FJG5