Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 2744844..2745346 Replicon chromosome
Accession NZ_CP010125
Organism Escherichia coli strain C8

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag RG30_RS14330 Protein ID WP_000767829.1
Coordinates 2744844..2745098 (-) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag RG30_RS14335 Protein ID WP_001259255.1
Coordinates 2745095..2745346 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
RG30_RS14305 2739859..2740086 - 228 WP_000985273.1 sulfurtransferase TusA family protein -
RG30_RS14310 2740100..2741158 - 1059 WP_000492346.1 transport protein YeeE -
RG30_RS14315 2741337..2742695 - 1359 WP_000019197.1 putrescine/proton symporter PlaP -
RG30_RS27335 2742685..2742747 - 63 WP_010723108.1 membrane protein YoeI -
RG30_RS14320 2742962..2743891 - 930 WP_000803351.1 LysR family transcriptional regulator -
RG30_RS14325 2743937..2744761 - 825 WP_000754758.1 SDR family oxidoreductase -
RG30_RS14330 2744844..2745098 - 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
RG30_RS14335 2745095..2745346 - 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
RG30_RS14340 2745630..2745680 + 51 WP_001364200.1 his operon leader peptide -
RG30_RS14350 2745826..2746725 + 900 WP_000131782.1 ATP phosphoribosyltransferase -
RG30_RS14355 2746731..2748035 + 1305 WP_001317939.1 histidinol dehydrogenase -
RG30_RS14360 2748032..2749102 + 1071 WP_000108941.1 histidinol-phosphate transaminase -
RG30_RS14365 2749102..2750169 + 1068 WP_000080060.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T50544 WP_000767829.1 NZ_CP010125:c2745098-2744844 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T50544 NZ_CP010125:c2745098-2744844 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT50544 WP_001259255.1 NZ_CP010125:c2745346-2745095 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT50544 NZ_CP010125:c2745346-2745095 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATATAACTCCCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATCATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References