Detailed information of TA system TA050531

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): ralRA/-
• Location: 1945079..1945450
• Replicon: chromosome
• Accession: NZ_CP010125
• Organism: Escherichia coli strain C8

Toxin (Protein)

• Gene name: ralR
• Uniprot ID: F4VC37
• Locus tag: RG30_RS10100
• Protein ID: WP_001317028.1
• Coordinates: 1945256..1945450 (-)
• Length: 65 a.a.

Antitoxin (RNA)

Gene name	ralA
Locus tag	-
Coordinates	1945079..1945257 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
RG30_RS27630	1940831..1941004	+	174	WP_001296046.1	hypothetical protein	-
RG30_RS10075	1941034..1942407	+	1374	WP_073533604.1	ATP-dependent RNA helicase DbpA	-
RG30_RS10080	1942536..1943471	-	936	WP_001157407.1	tRNA 2-thiocytidine(32) synthetase TtcA	-
RG30_RS10085	1943523..1944758	-	1236	WP_000040852.1	site-specific integrase	-
RG30_RS10090	1944760..1944975	-	216	WP_000079604.1	excisionase XisR	-
RG30_RS10095	1945054..1945263	-	210	WP_021565074.1	double-strand break reduction protein RcbA	-
-	1945079..1945257	+	179	NuclAT_0	-	Antitoxin
-	1945079..1945257	+	179	NuclAT_0	-	Antitoxin
-	1945079..1945257	+	179	NuclAT_0	-	Antitoxin
-	1945079..1945257	+	179	NuclAT_0	-	Antitoxin
RG30_RS10100	1945256..1945450	-	195	WP_001317028.1	type I toxin-antitoxin system endodeoxyribonuclease toxin RalR	Toxin
RG30_RS10105	1945507..1946316	-	810	WP_073533605.1	recombination protein RecT	-
RG30_RS10110	1946309..1948908	-	2600	Protein_1826	exodeoxyribonuclease VIII	-
RG30_RS10115	1949010..1949285	-	276	WP_000632297.1	protein RacC	-
RG30_RS10120	1949360..1949530	-	171	WP_077899810.1	hypothetical protein	-
RG30_RS10125	1949530..1949751	-	222	WP_000560223.1	killing protein KilR	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1936264..1991667	55403

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Endodeoxyribonuclease toxin RalR (6-54)

Sequences

Toxin

Protein

• Length: 65 a.a.
• Molecular weight: 7018.89 Da
• Isoelectric Point: 8.9538

>T50531 WP_001317028.1 NZ_CP010125:c1945450-1945256 [Escherichia coli]
MRYDNVKPCPFCGCPSVTVKAISGYYRAKCNGCESRTGYGGSEKEALERWNKRTTGNNNGGVHV

Nucleotide

• Length: 195 bp

>T50531 NZ_CP010125:c1945450-1945256 [Escherichia coli]
ATGAGATATGACAATGTTAAACCATGTCCATTTTGTGGTTGTCCATCAGTAACGGTGAAAGCCATTTCAGGATATTACCG
AGCGAAGTGTAACGGATGCGAATCCCGAACCGGTTATGGTGGAAGTGAAAAAGAAGCACTCGAAAGATGGAATAAACGAA
CCACTGGAAATAATAATGGAGGTGTTCATGTATAA

Antitoxin

Nucleotide

• Length: 179 bp

>AT50531 NZ_CP010125:1945079-1945257 [Escherichia coli]
GAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGA
GAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGAAGTGCCACCTTCCTTTT
CAATAGTGGCGGTAATTTT

Structures

Toxin

• AlphaFold DB: A0A7L6LHV7

Antitoxin