Detailed information of TA system TA050521

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 4541..4920
• Replicon: plasmid B
• Accession: NZ_CP010124
• Organism: Escherichia coli strain C5

Toxin (Protein)

• Gene name: hok
• Uniprot ID: -
• Locus tag: RG28_RS29650
• Protein ID: WP_073521365.1
• Coordinates: 4741..4920 (+)
• Length: 60 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	4541..4675 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
RG28_RS32030	35..130	+	96	WP_073521414.1	chromosome partitioning protein ParB	-
RG28_RS29615	185..619	+	435	WP_061361213.1	conjugation system SOS inhibitor PsiB	-
RG28_RS29620	616..1335	+	720	WP_061361214.1	plasmid SOS inhibition protein A	-
RG28_RS29625	1335..1691	+	357	Protein_4	hypothetical protein	-
RG28_RS29630	1720..3291	-	1572	WP_073521364.1	IS66 family transposase	-
RG28_RS29635	3311..3658	-	348	WP_000624618.1	IS66 family insertion sequence element accessory protein TnpB	-
RG28_RS29640	3658..4308	-	651	WP_061354651.1	IS66 family insertion sequence hypothetical protein	-
-	4541..4675	+	135	NuclAT_0	-	Antitoxin
-	4541..4675	+	135	NuclAT_0	-	Antitoxin
-	4541..4675	+	135	NuclAT_0	-	Antitoxin
-	4541..4675	+	135	NuclAT_0	-	Antitoxin
RG28_RS29650	4741..4920	+	180	WP_073521365.1	type I toxin-antitoxin system Hok family toxin	Toxin
RG28_RS29660	5485..7164	+	1680	WP_077897899.1	group II intron reverse transcriptase/maturase	-
RG28_RS29675	7922..8128	+	207	WP_061361210.1	hypothetical protein	-
RG28_RS29680	8152..8448	+	297	WP_001272251.1	hypothetical protein	-
RG28_RS29685	8557..9378	+	822	WP_001234447.1	DUF945 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..86032	86032

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (7-48)

Sequences

Toxin

Protein

• Length: 60 a.a.
• Molecular weight: 6785.20 Da
• Isoelectric Point: 8.3506

>T50521 WP_073521365.1 NZ_CP010124:4741-4920 [Escherichia coli]
MKLPGNALIWCVLIVCCTLLIFTLLTRNRLCEVRLKDGYREVTATMAYECDKKSSNILF

Nucleotide

• Length: 180 bp

>T50521 NZ_CP010124:4741-4920 [Escherichia coli]
ATGAAACTACCGGGAAACGCCCTTATCTGGTGCGTATTAATCGTGTGCTGCACGTTGTTGATATTCACACTCCTGACCCG
GAATCGTCTGTGCGAAGTCCGGCTGAAGGACGGATACAGGGAAGTTACGGCAACAATGGCTTACGAGTGCGACAAGAAGT
CGTCCAACATATTGTTTTGA

Antitoxin

Nucleotide

• Length: 135 bp

>AT50521 NZ_CP010124:4541-4675 [Escherichia coli]
CAGGGTGCAGATATATGGGCTATGATGTGTCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTACCGT
GTTGTGTGGCAGAAGGCAGAAAGCCCCGTAGTTAATTTTCATTAACCCACGAGGC

Structures

Antitoxin