50517

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 94632..95493 Replicon plasmid A
Accession NZ_CP010123
Organism Escherichia coli strain C5

Toxin (Protein)


Gene name yhaV Uniprot ID A0A7W3G1F8
Locus tag RG28_RS28895 Protein ID WP_024195926.1
Coordinates 94632..95126 (-) Length 165 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID A0A7W3G1H0
Locus tag RG28_RS28900 Protein ID WP_001559862.1
Coordinates 95149..95493 (-) Length 115 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
RG28_RS28890 90644..94450 - 3807 WP_001559860.1 hypothetical protein -
RG28_RS28895 94632..95126 - 495 WP_024195926.1 type II toxin-antitoxin system YhaV family toxin Toxin
RG28_RS28900 95149..95493 - 345 WP_001559862.1 type II toxin-antitoxin system PrlF family antitoxin Antitoxin
RG28_RS28905 95737..95814 - 78 Protein_116 helix-turn-helix domain-containing protein -
RG28_RS28910 96075..96305 + 231 WP_001604445.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein -
RG28_RS28915 96302..96718 + 417 WP_047612429.1 type II toxin-antitoxin system VapC family toxin -
RG28_RS28920 96858..97838 + 981 WP_073521305.1 hypothetical protein -
RG28_RS28925 98050..99621 - 1572 WP_047613524.1 ATP-binding protein -
RG28_RS28930 99940..100209 + 270 WP_073521306.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - pefD / pefC / pefA / papD / papC 1..214445 214445


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(11-147)

Antitoxin

PrlF antitoxin

(16-114)


Sequences

Toxin        


Download         Length: 165 a.a.        Molecular weight: 19275.36 Da        Isoelectric Point: 8.7864

>T50517 WP_024195926.1 NZ_CP010123:c95126-94632 [Escherichia coli]
MEYLEINGWKICFHECFLRQITGLMKKVAELKASKPLEYHKKKETKLLVAIERVIEERITANPLDPQFRQGNTLGEEYKH
WFRAKFLMQFRLFYRCSQEHKTIVIGWVNGFDTLRAYDSRTDAYKVFGEMLKAGNPPDDWDTLLCEAGKATATTNMVDFL
TKRI

Download         Length: 495 bp

>T50517 NZ_CP010123:c95126-94632 [Escherichia coli]
ATGGAATACTTAGAAATAAACGGTTGGAAAATCTGCTTTCACGAGTGTTTTTTAAGGCAGATTACAGGATTAATGAAGAA
GGTGGCAGAGTTAAAGGCTTCTAAACCACTGGAATATCATAAGAAAAAGGAAACTAAACTTCTTGTTGCGATCGAGCGTG
TTATAGAGGAAAGGATAACCGCTAACCCGCTTGATCCACAGTTTCGGCAGGGGAATACTTTAGGCGAAGAATACAAACAC
TGGTTCCGGGCAAAATTTTTGATGCAGTTCCGTTTGTTTTATCGTTGTAGCCAGGAACATAAAACAATTGTTATTGGCTG
GGTAAATGGATTTGACACGCTTCGTGCTTATGATTCCAGGACGGATGCCTATAAGGTATTTGGTGAAATGTTGAAAGCTG
GAAATCCACCGGATGACTGGGATACCCTTCTCTGTGAGGCTGGTAAAGCTACAGCGACCACCAATATGGTTGATTTTCTC
ACAAAGCGTATTTGA

Antitoxin


Download         Length: 115 a.a.        Molecular weight: 12755.59 Da        Isoelectric Point: 4.6652

>AT50517 WP_001559862.1 NZ_CP010123:c95493-95149 [Escherichia coli]
MATQILNSAEVSLRAESRLTERSQTTIPAVIRDALHLKPGEYIHYTLLAGGKVIMSRQNEEQDDPVISQFLTFLENDMKK
NPQKIAPVPTMFWESMKALTAGINVDLDAPLTED

Download         Length: 345 bp

>AT50517 NZ_CP010123:c95493-95149 [Escherichia coli]
ATGGCTACTCAAATTTTAAATAGTGCAGAAGTGTCACTGCGTGCAGAGTCACGACTTACTGAACGCAGTCAGACGACGAT
CCCGGCAGTAATACGCGATGCTTTGCATCTCAAGCCCGGTGAGTATATTCATTACACTTTACTTGCAGGTGGTAAGGTCA
TCATGTCACGACAGAATGAAGAACAGGACGACCCTGTGATTTCGCAGTTTTTGACTTTTCTTGAAAACGACATGAAAAAG
AACCCACAGAAGATTGCTCCAGTTCCGACAATGTTCTGGGAAAGCATGAAAGCATTAACTGCCGGAATTAATGTTGATTT
AGACGCACCACTTACCGAAGATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7W3G1F8

Antitoxin

Source ID Structure
AlphaFold DB A0A7W3G1H0

References