Detailed information of TA system TA050512

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 66708..67068
• Replicon: plasmid A
• Accession: NZ_CP010123
• Organism: Escherichia coli strain C5

Toxin (Protein)

• Gene name: hok
• Uniprot ID: -
• Locus tag: RG28_RS28690
• Protein ID: WP_073521290.1
• Coordinates: 66708..66869 (-)
• Length: 54 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	66915..67068 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
RG28_RS28640	61780..62007	-	228	WP_001254386.1	conjugal transfer relaxosome protein TraY	-
RG28_RS28645	62101..62787	-	687	WP_073521287.1	PAS domain-containing protein	-
RG28_RS28650	62974..63357	-	384	WP_073521288.1	relaxosome protein TraM	-
RG28_RS28655	63634..64281	+	648	Protein_75	transglycosylase SLT domain-containing protein	-
RG28_RS28660	64578..65399	-	822	WP_001234447.1	DUF945 domain-containing protein	-
RG28_RS28665	65508..65804	-	297	WP_001272251.1	hypothetical protein	-
RG28_RS28670	65828..66034	-	207	WP_000547959.1	hypothetical protein	-
RG28_RS31990	66104..66462	+	359	Protein_79	hypothetical protein	-
RG28_RS28690	66708..66869	-	162	WP_073521290.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	66915..67068	-	154	NuclAT_0	-	Antitoxin
-	66915..67068	-	154	NuclAT_0	-	Antitoxin
-	66915..67068	-	154	NuclAT_0	-	Antitoxin
-	66915..67068	-	154	NuclAT_0	-	Antitoxin
RG28_RS28700	67044..67562	-	519	WP_073521356.1	hypothetical protein	-
RG28_RS28705	67562..68281	-	720	WP_073521291.1	plasmid SOS inhibition protein A	-
RG28_RS28710	68278..68712	-	435	WP_050940544.1	conjugation system SOS inhibitor PsiB	-
RG28_RS28715	68781..70745	-	1965	WP_073521292.1	ParB/RepB/Spo0J family partition protein	-
RG28_RS28720	70811..71044	-	234	WP_001705780.1	DUF905 domain-containing protein	-
RG28_RS28725	71102..71650	-	549	WP_073521355.1	single-stranded DNA-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	pefD / pefC / pefA / papD / papC	1..214445	214445

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (7-48)

Sequences

Toxin

Protein

• Length: 54 a.a.
• Molecular weight: 6008.31 Da
• Isoelectric Point: 8.5101

>T50512 WP_073521290.1 NZ_CP010123:c66869-66708 [Escherichia coli]
MKLPGNALIWCVLIVCCTLLIFTLLTRNRLCEVRLKDGYREVMATMAYESGGK

Nucleotide

• Length: 162 bp

>T50512 NZ_CP010123:c66869-66708 [Escherichia coli]
ATGAAACTACCGGGAAACGCCCTTATCTGGTGCGTATTAATCGTGTGCTGCACGTTGTTAATATTCACACTCCTGACCCG
GAATCGCCTGTGCGAAGTCCGGCTGAAGGACGGATACAGGGAAGTTATGGCAACAATGGCTTACGAATCCGGCGGTAAGT
AG

Antitoxin

Nucleotide

• Length: 154 bp

>AT50512 NZ_CP010123:c67068-66915 [Escherichia coli]
CAGGGTGCAGATATATGGGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTATCCT
GTCGTGTGGCAGAAGGACAAAAGCCCCGTAGTTAATTTTCATTAACCCACGAGGCCTCTGCATGTCTAGTCCAC

Structures

Antitoxin