Detailed information of TA system TA050510

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 27069..27323
• Replicon: plasmid A
• Accession: NZ_CP010123
• Organism: Escherichia coli strain C5

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: -
• Locus tag: RG28_RS28430
• Protein ID: WP_040089444.1
• Coordinates: 27069..27275 (-)
• Length: 69 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	27262..27323 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
RG28_RS28395	23562..24584	-	1023	WP_042973996.1	replication initiation protein	-
RG28_RS28400	24574..25107	-	534	WP_040089446.1	replication protein	-
RG28_RS28405	25100..25174	-	75	WP_001367777.1	RepA leader peptide Tap	-
RG28_RS28415	25408..25665	-	258	WP_001136637.1	replication regulatory protein RepA	-
RG28_RS28420	25973..26455	-	483	WP_000405250.1	GNAT family N-acetyltransferase	-
RG28_RS28425	26446..26748	-	303	WP_072146426.1	DUF1778 domain-containing protein	-
RG28_RS28430	27069..27275	-	207	WP_040089444.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	27262..27323	+	62	NuclAT_1	-	Antitoxin
-	27262..27323	+	62	NuclAT_1	-	Antitoxin
-	27262..27323	+	62	NuclAT_1	-	Antitoxin
-	27262..27323	+	62	NuclAT_1	-	Antitoxin
RG28_RS28440	27564..27773	-	210	WP_073521270.1	hemolysin expression modulator Hha	-
RG28_RS28445	27830..28066	-	237	WP_061358569.1	hypothetical protein	-
RG28_RS28450	28313..28873	-	561	WP_073521271.1	fertility inhibition protein FinO	-
RG28_RS28455	28928..29671	-	744	WP_073521272.1	conjugal transfer pilus acetylase TraX	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	pefD / pefC / pefA / papD / papC	1..214445	214445

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (23-64)

Sequences

Toxin

Protein

• Length: 69 a.a.
• Molecular weight: 7820.35 Da
• Isoelectric Point: 8.8807

>T50510 WP_040089444.1 NZ_CP010123:c27275-27069 [Escherichia coli]
MKYLNTTDCSLFLAERSKFMTKYALIGLLAVCATLLCFSLIFRERLCELNIHRGNTVVQITLAYEARK

Nucleotide

• Length: 207 bp

>T50510 NZ_CP010123:c27275-27069 [Escherichia coli]
ATGAAGTACCTTAACACTACTGATTGTAGCCTCTTCCTTGCAGAGAGGTCAAAGTTTATGACGAAATATGCCCTTATAGG
GTTGCTCGCCGTGTGCGCCACGTTGTTGTGTTTTTCACTGATATTCAGGGAACGGTTGTGTGAGCTGAATATTCACAGGG
GAAATACAGTGGTGCAGATAACTCTGGCCTACGAAGCACGGAAGTAA

Antitoxin

Nucleotide

• Length: 62 bp

>AT50510 NZ_CP010123:27262-27323 [Escherichia coli]
TTAAGGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Antitoxin