Detailed information of TA system TA050505

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4909540..4910142
• Replicon: chromosome
• Accession: NZ_CP010122
• Organism: Escherichia coli strain C5

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: RG28_RS26025
• Protein ID: WP_000897305.1
• Coordinates: 4909540..4909851 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: RG28_RS26030
• Protein ID: WP_000356397.1
• Coordinates: 4909852..4910142 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
RG28_RS25995	4904570..4905355	+	786	WP_000059678.1	DeoR/GlpR family DNA-binding transcription regulator	-
RG28_RS26000	4905454..4906053	+	600	WP_001295269.1	glucose-1-phosphatase	-
RG28_RS26005	4906047..4906919	+	873	WP_000920762.1	virulence factor BrkB family protein	-
RG28_RS26010	4906916..4907353	+	438	WP_000560983.1	D-tyrosyl-tRNA(Tyr) deacylase	-
RG28_RS26015	4907398..4908339	+	942	WP_001343389.1	fatty acid biosynthesis protein FabY	-
RG28_RS26020	4908403..4909311	-	909	WP_001162704.1	alpha/beta hydrolase	-
RG28_RS26025	4909540..4909851	+	312	WP_000897305.1	hypothetical protein	Toxin
RG28_RS26030	4909852..4910142	+	291	WP_000356397.1	helix-turn-helix domain-containing protein	Antitoxin
RG28_RS26040	4910747..4910965	+	219	WP_001297075.1	ribbon-helix-helix domain-containing protein	-
RG28_RS26045	4911184..4911426	+	243	WP_001086388.1	hypothetical protein	-
RG28_RS26055	4911756..4912685	-	930	WP_000027708.1	formate dehydrogenase accessory protein FdhE	-
RG28_RS26060	4912682..4913317	-	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
RG28_RS26065	4913314..4914216	-	903	WP_000331377.1	formate dehydrogenase O subunit beta	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T50505 WP_000897305.1 NZ_CP010122:4909540-4909851 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T50505 NZ_CP010122:4909540-4909851 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTTTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT50505 WP_000356397.1 NZ_CP010122:4909852-4910142 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT50505 NZ_CP010122:4909852-4910142 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
GCGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCTGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7