Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | higBA (relBE)/Tad-HTH_37 |
| Location | 3795828..3796455 | Replicon | chromosome |
| Accession | NZ_CP010122 | ||
| Organism | Escherichia coli strain C5 | ||
Toxin (Protein)
| Gene name | higB | Uniprot ID | - |
| Locus tag | RG28_RS20200 | Protein ID | WP_072301615.1 |
| Coordinates | 3795828..3796157 (+) | Length | 110 a.a. |
Antitoxin (Protein)
| Gene name | higA | Uniprot ID | - |
| Locus tag | RG28_RS20205 | Protein ID | WP_000147080.1 |
| Coordinates | 3796147..3796455 (+) | Length | 103 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| RG28_RS20170 | 3790991..3792391 | - | 1401 | WP_061351023.1 | replicative DNA helicase | - |
| RG28_RS20175 | 3792388..3793266 | - | 879 | WP_000988199.1 | ATP-binding protein | - |
| RG28_RS20180 | 3793277..3794119 | - | 843 | WP_052773596.1 | hypothetical protein | - |
| RG28_RS20185 | 3794251..3794475 | - | 225 | WP_047089426.1 | hypothetical protein | - |
| RG28_RS20190 | 3794472..3795338 | - | 867 | WP_171840557.1 | Rha family phage regulatory protein | - |
| RG28_RS20195 | 3795423..3795713 | + | 291 | WP_157916595.1 | SWIM zinc finger family protein | - |
| RG28_RS20200 | 3795828..3796157 | + | 330 | WP_072301615.1 | type II toxin-antitoxin system RelE/ParE family toxin | Toxin |
| RG28_RS20205 | 3796147..3796455 | + | 309 | WP_000147080.1 | helix-turn-helix transcriptional regulator | Antitoxin |
| RG28_RS20210 | 3796474..3796650 | - | 177 | WP_001197746.1 | DUF4222 domain-containing protein | - |
| RG28_RS20215 | 3796670..3797377 | - | 708 | WP_061352046.1 | Rha family transcriptional regulator | - |
| RG28_RS20220 | 3797377..3797688 | - | 312 | WP_000932280.1 | hypothetical protein | - |
| RG28_RS20225 | 3797764..3798084 | - | 321 | WP_000186943.1 | hypothetical protein | - |
| RG28_RS20230 | 3798173..3798400 | + | 228 | WP_000132761.1 | hypothetical protein | - |
| RG28_RS20235 | 3798522..3798794 | - | 273 | WP_057697517.1 | helix-turn-helix domain-containing protein | - |
| RG28_RS20240 | 3798928..3799617 | + | 690 | WP_057697516.1 | LexA family transcriptional regulator | - |
| RG28_RS20245 | 3799763..3800224 | + | 462 | WP_072301616.1 | helix-turn-helix transcriptional regulator | - |
| RG28_RS20250 | 3800380..3800586 | + | 207 | WP_057698620.1 | hypothetical protein | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Integrative and Conjugative Element | - | gspM / gspL / gspK / gspJ / gspI / gspH / gspG / gspF / gspE / gspD / gspC | 3788869..3893303 | 104434 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 110 a.a. Molecular weight: 12779.78 Da Isoelectric Point: 9.8425
>T50500 WP_072301615.1 NZ_CP010122:3795828-3796157 [Escherichia coli]
MNYTIEYYSEEVRLEVDQLPLSMRARYQHLVERMKIYGSNLGEPHTSAFGNGLFELRIKGSDGIARVFYCTLAGKRIIML
HSFIKKTQKTPPAERQKAETRMKEVKHDW
MNYTIEYYSEEVRLEVDQLPLSMRARYQHLVERMKIYGSNLGEPHTSAFGNGLFELRIKGSDGIARVFYCTLAGKRIIML
HSFIKKTQKTPPAERQKAETRMKEVKHDW
Download Length: 330 bp
>T50500 NZ_CP010122:3795828-3796157 [Escherichia coli]
ATGAACTACACTATCGAATACTACAGTGAAGAGGTCAGGCTGGAGGTCGACCAGCTTCCATTGAGTATGCGTGCCCGATA
CCAGCATCTTGTTGAACGCATGAAGATATACGGCAGCAATCTCGGAGAACCTCATACCAGTGCTTTCGGTAACGGGCTTT
TCGAGCTTCGGATTAAAGGTAGTGATGGCATCGCACGCGTCTTTTACTGCACCCTGGCAGGAAAACGCATCATCATGCTG
CATAGTTTCATCAAGAAGACCCAGAAAACCCCACCAGCCGAACGCCAAAAAGCTGAAACCAGAATGAAGGAGGTAAAGCA
TGACTGGTAA
ATGAACTACACTATCGAATACTACAGTGAAGAGGTCAGGCTGGAGGTCGACCAGCTTCCATTGAGTATGCGTGCCCGATA
CCAGCATCTTGTTGAACGCATGAAGATATACGGCAGCAATCTCGGAGAACCTCATACCAGTGCTTTCGGTAACGGGCTTT
TCGAGCTTCGGATTAAAGGTAGTGATGGCATCGCACGCGTCTTTTACTGCACCCTGGCAGGAAAACGCATCATCATGCTG
CATAGTTTCATCAAGAAGACCCAGAAAACCCCACCAGCCGAACGCCAAAAAGCTGAAACCAGAATGAAGGAGGTAAAGCA
TGACTGGTAA
Antitoxin
Download Length: 103 a.a. Molecular weight: 11238.90 Da Isoelectric Point: 7.0161
>AT50500 WP_000147080.1 NZ_CP010122:3796147-3796455 [Escherichia coli]
MTGKRNPPTITHDEMADKWMEDPAFKAEYDAIADEFALLDEMLAARKEAGLTQAEIAERMGTKATVISRMESNLASGVSG
PSFSTLKKFARATGKKLQIRFV
MTGKRNPPTITHDEMADKWMEDPAFKAEYDAIADEFALLDEMLAARKEAGLTQAEIAERMGTKATVISRMESNLASGVSG
PSFSTLKKFARATGKKLQIRFV
Download Length: 309 bp
>AT50500 NZ_CP010122:3796147-3796455 [Escherichia coli]
ATGACTGGTAAACGTAATCCGCCAACCATTACACATGATGAAATGGCTGATAAATGGATGGAGGATCCAGCTTTTAAAGC
AGAATACGACGCCATCGCCGACGAATTTGCGTTGCTTGATGAAATGCTGGCAGCACGTAAAGAAGCTGGTTTAACCCAGG
CTGAGATCGCTGAGCGTATGGGAACCAAAGCAACTGTCATCAGCAGAATGGAAAGTAATCTGGCGTCAGGTGTCAGTGGA
CCATCATTTTCCACACTGAAAAAATTTGCCCGCGCAACAGGAAAAAAACTCCAGATCCGCTTCGTGTAA
ATGACTGGTAAACGTAATCCGCCAACCATTACACATGATGAAATGGCTGATAAATGGATGGAGGATCCAGCTTTTAAAGC
AGAATACGACGCCATCGCCGACGAATTTGCGTTGCTTGATGAAATGCTGGCAGCACGTAAAGAAGCTGGTTTAACCCAGG
CTGAGATCGCTGAGCGTATGGGAACCAAAGCAACTGTCATCAGCAGAATGGAAAGTAATCTGGCGTCAGGTGTCAGTGGA
CCATCATTTTCCACACTGAAAAAATTTGCCCGCGCAACAGGAAAAAAACTCCAGATCCGCTTCGTGTAA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
Structures
Toxin
| Source | ID | Structure |
|---|
Antitoxin
| Source | ID | Structure |
|---|