50488

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 2411610..2412112 Replicon chromosome
Accession NZ_CP010122
Organism Escherichia coli strain C5

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag RG28_RS12430 Protein ID WP_000767829.1
Coordinates 2411858..2412112 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag RG28_RS12425 Protein ID WP_001259255.1
Coordinates 2411610..2411861 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
RG28_RS12395 2406788..2407855 - 1068 WP_000080093.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -
RG28_RS12400 2407855..2408925 - 1071 WP_000108972.1 histidinol-phosphate transaminase -
RG28_RS12405 2408922..2410226 - 1305 WP_021293346.1 histidinol dehydrogenase -
RG28_RS12410 2410232..2411131 - 900 WP_000131782.1 ATP phosphoribosyltransferase -
RG28_RS12420 2411277..2411327 - 51 WP_001364200.1 his operon leader peptide -
RG28_RS12425 2411610..2411861 + 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
RG28_RS12430 2411858..2412112 + 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
RG28_RS12435 2412195..2413019 + 825 WP_000754737.1 SDR family oxidoreductase -
RG28_RS12440 2413065..2413994 + 930 WP_073520974.1 LysR family transcriptional regulator -
RG28_RS31215 2414209..2414271 + 63 WP_010723108.1 membrane protein YoeI -
RG28_RS12445 2414261..2415619 + 1359 WP_000019197.1 putrescine/proton symporter PlaP -
RG28_RS12450 2415798..2416856 + 1059 WP_000492340.1 transport protein YeeE -
RG28_RS12455 2416870..2417097 + 228 WP_000985273.1 sulfurtransferase TusA family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T50488 WP_000767829.1 NZ_CP010122:2411858-2412112 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T50488 NZ_CP010122:2411858-2412112 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT50488 WP_001259255.1 NZ_CP010122:2411610-2411861 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT50488 NZ_CP010122:2411610-2411861 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References