Detailed information of TA system TA050486

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/Tad-HTH_37
• Location: 1703316..1703943
• Replicon: chromosome
• Accession: NZ_CP010122
• Organism: Escherichia coli strain C5

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: RG28_RS08895
• Protein ID: WP_072301615.1
• Coordinates: 1703614..1703943 (-)
• Length: 110 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: RG28_RS08890
• Protein ID: WP_000147080.1
• Coordinates: 1703316..1703624 (-)
• Length: 103 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
RG28_RS08845	1699185..1699391	-	207	WP_057698620.1	hypothetical protein	-
RG28_RS08850	1699547..1700008	-	462	WP_072301616.1	helix-turn-helix transcriptional regulator	-
RG28_RS08855	1700154..1700843	-	690	WP_057697516.1	LexA family transcriptional regulator	-
RG28_RS08860	1700977..1701249	+	273	WP_057697517.1	helix-turn-helix domain-containing protein	-
RG28_RS08865	1701371..1701598	-	228	WP_000132761.1	hypothetical protein	-
RG28_RS08870	1701687..1702007	+	321	WP_000186943.1	hypothetical protein	-
RG28_RS08875	1702083..1702394	+	312	WP_000932280.1	hypothetical protein	-
RG28_RS08880	1702394..1703101	+	708	WP_061352046.1	Rha family transcriptional regulator	-
RG28_RS08885	1703121..1703297	+	177	WP_001197746.1	DUF4222 domain-containing protein	-
RG28_RS08890	1703316..1703624	-	309	WP_000147080.1	helix-turn-helix transcriptional regulator	Antitoxin
RG28_RS08895	1703614..1703943	-	330	WP_072301615.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
RG28_RS08900	1704058..1704348	-	291	WP_157916595.1	SWIM zinc finger family protein	-
RG28_RS08905	1704433..1705299	+	867	WP_171840557.1	Rha family phage regulatory protein	-
RG28_RS08910	1705296..1705520	+	225	WP_047089426.1	hypothetical protein	-
RG28_RS08915	1705652..1706494	+	843	WP_052773596.1	hypothetical protein	-
RG28_RS08920	1706505..1707383	+	879	WP_000988199.1	ATP-binding protein	-
RG28_RS08925	1707380..1708780	+	1401	WP_061351023.1	replicative DNA helicase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1670549..1741401	70852

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (16-103)

Antitoxin

• Cro/C1-type helix-turn-helix domain (44-97)

Sequences

Toxin

Protein

• Length: 110 a.a.
• Molecular weight: 12779.78 Da
• Isoelectric Point: 9.8425

>T50486 WP_072301615.1 NZ_CP010122:c1703943-1703614 [Escherichia coli]
MNYTIEYYSEEVRLEVDQLPLSMRARYQHLVERMKIYGSNLGEPHTSAFGNGLFELRIKGSDGIARVFYCTLAGKRIIML
HSFIKKTQKTPPAERQKAETRMKEVKHDW

Nucleotide

• Length: 330 bp

>T50486 NZ_CP010122:c1703943-1703614 [Escherichia coli]
ATGAACTACACTATCGAATACTACAGTGAAGAGGTCAGGCTGGAGGTCGACCAGCTTCCATTGAGTATGCGTGCCCGATA
CCAGCATCTTGTTGAACGCATGAAGATATACGGCAGCAATCTCGGAGAACCTCATACCAGTGCTTTCGGTAACGGGCTTT
TCGAGCTTCGGATTAAAGGTAGTGATGGCATCGCACGCGTCTTTTACTGCACCCTGGCAGGAAAACGCATCATCATGCTG
CATAGTTTCATCAAGAAGACCCAGAAAACCCCACCAGCCGAACGCCAAAAAGCTGAAACCAGAATGAAGGAGGTAAAGCA
TGACTGGTAA

Antitoxin

Protein

• Length: 103 a.a.
• Molecular weight: 11238.90 Da
• Isoelectric Point: 7.0161

>AT50486 WP_000147080.1 NZ_CP010122:c1703624-1703316 [Escherichia coli]
MTGKRNPPTITHDEMADKWMEDPAFKAEYDAIADEFALLDEMLAARKEAGLTQAEIAERMGTKATVISRMESNLASGVSG
PSFSTLKKFARATGKKLQIRFV

Nucleotide

• Length: 309 bp

>AT50486 NZ_CP010122:c1703624-1703316 [Escherichia coli]
ATGACTGGTAAACGTAATCCGCCAACCATTACACATGATGAAATGGCTGATAAATGGATGGAGGATCCAGCTTTTAAAGC
AGAATACGACGCCATCGCCGACGAATTTGCGTTGCTTGATGAAATGCTGGCAGCACGTAAAGAAGCTGGTTTAACCCAGG
CTGAGATCGCTGAGCGTATGGGAACCAAAGCAACTGTCATCAGCAGAATGGAAAGTAATCTGGCGTCAGGTGTCAGTGGA
CCATCATTTTCCACACTGAAAAAATTTGCCCGCGCAACAGGAAAAAAACTCCAGATCCGCTTCGTGTAA