Detailed information of TA system TA050473

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 4166141..4166835
• Replicon: chromosome
• Accession: NZ_CP010121
• Organism: Escherichia coli strain C4

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: V0YKG6
• Locus tag: RG27_RS21480
• Protein ID: WP_001263485.1
• Coordinates: 4166141..4166539 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1QAE3
• Locus tag: RG27_RS21485
• Protein ID: WP_000554758.1
• Coordinates: 4166542..4166835 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
RG27_RS21450	4161310..4162554	-	1245	WP_000189541.1	esterase FrsA	-
-	4161970..4162050	-	81	NuclAT_9	-	-
-	4161970..4162050	-	81	NuclAT_9	-	-
-	4161970..4162050	-	81	NuclAT_9	-	-
-	4161970..4162050	-	81	NuclAT_9	-	-
RG27_RS21455	4162646..4163104	-	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
RG27_RS21460	4163365..4164822	+	1458	WP_001293009.1	cytosol nonspecific dipeptidase	-
RG27_RS26710	4164879..4165231	-	353	Protein_3894	peptide chain release factor H	-
RG27_RS21470	4165227..4165433	-	207	Protein_3895	RtcB family protein	-
RG27_RS21475	4165679..4166131	-	453	WP_001059899.1	GNAT family N-acetyltransferase	-
RG27_RS21480	4166141..4166539	-	399	WP_001263485.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
RG27_RS21485	4166542..4166835	-	294	WP_000554758.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
RG27_RS21490	4166887..4167942	-	1056	WP_001226177.1	DNA polymerase IV	-
RG27_RS21495	4168013..4168798	-	786	WP_000207554.1	putative lateral flagellar export/assembly protein LafU	-
RG27_RS21500	4168770..4170482	+	1713	Protein_3901	flagellar biosynthesis protein FlhA	-
RG27_RS21520	4170921..4171418	-	498	WP_000006263.1	REP-associated tyrosine transposase RayT	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15418.84 Da
• Isoelectric Point: 7.3840

>T50473 WP_001263485.1 NZ_CP010121:c4166539-4166141 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGILPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLCQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T50473 NZ_CP010121:c4166539-4166141 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTATTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGTGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGTGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAGCCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGAAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.76 Da
• Isoelectric Point: 5.2170

>AT50473 WP_000554758.1 NZ_CP010121:c4166835-4166542 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT50473 NZ_CP010121:c4166835-4166542 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCGAGTGCTGCAAGATTAGAGGAAATTACACGCCGCGCT
GAACAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Structures

Toxin

• AlphaFold DB: V0YKG6

Antitoxin

• AlphaFold DB: S1QAE3