Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 2758910..2759472 Replicon chromosome
Accession NZ_CP010121
Organism Escherichia coli strain C4

Toxin (Protein)


Gene name higB Uniprot ID L4J948
Locus tag RG27_RS14140 Protein ID WP_000605676.1
Coordinates 2758910..2759188 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID S1PAQ1
Locus tag RG27_RS14145 Protein ID WP_000781370.1
Coordinates 2759188..2759472 (+) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
RG27_RS14110 2754496..2754927 - 432 WP_000152305.1 peroxiredoxin OsmC -
RG27_RS14120 2755272..2755487 + 216 WP_000495766.1 biofilm-dependent modulation protein -
RG27_RS14125 2755589..2755726 + 138 WP_000841554.1 stationary-phase-induced ribosome-associated protein -
RG27_RS14130 2755883..2757580 + 1698 WP_000433464.1 malate dehydrogenase -
RG27_RS14135 2757714..2758724 + 1011 WP_053296092.1 alcohol dehydrogenase AdhP -
RG27_RS14140 2758910..2759188 + 279 WP_000605676.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
RG27_RS14145 2759188..2759472 + 285 WP_000781370.1 HigA family addiction module antidote protein Antitoxin
RG27_RS14150 2759524..2760177 - 654 WP_000045646.1 formate dehydrogenase-N subunit gamma -
RG27_RS14155 2760170..2761054 - 885 WP_001240582.1 formate dehydrogenase N subunit beta -
RG27_RS14160 2761067..2764114 - 3048 WP_077815972.1 formate dehydrogenase-N subunit alpha -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(4-92)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(1-88)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10521.10 Da        Isoelectric Point: 7.3562

>T50467 WP_000605676.1 NZ_CP010121:2758910-2759188 [Escherichia coli]
MIMNFRHKGLRDLFLLGKTSGVIPTQVKRLRHRLAVIDAASCLADIDMPGYRLHPLSGDRDGIWAISVSGNWRITFEFVN
GDAYILDYEDYH

Download         Length: 279 bp

>T50467 NZ_CP010121:2758910-2759188 [Escherichia coli]
ATGATAATGAATTTCAGACACAAGGGATTGCGTGACTTGTTTCTTCTCGGCAAAACCTCCGGCGTTATACCGACACAAGT
CAAACGATTACGCCACCGACTCGCTGTGATTGATGCAGCAAGCTGCCTTGCTGATATCGATATGCCCGGTTACCGACTAC
ACCCATTAAGCGGTGATCGCGATGGAATTTGGGCGATATCTGTCTCGGGCAACTGGCGAATCACATTTGAATTCGTCAAT
GGCGATGCATACATACTGGATTACGAGGACTATCACTAA

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10401.08 Da        Isoelectric Point: 8.8567

>AT50467 WP_000781370.1 NZ_CP010121:2759188-2759472 [Escherichia coli]
MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTGKAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAE
KTVDVSRLRRLVTQ

Download         Length: 285 bp

>AT50467 NZ_CP010121:2759188-2759472 [Escherichia coli]
ATGAAAATGGCCAATCATCCCCGCCCGGGGGACATTATTCAGGAATCACTGGACGAACTTAATGTCAGCCTGCGCGAGTT
TGCCAGAGCAATGGAAATTGCGCCCTCAACGGCAAGCCGATTGCTGACCGGAAAAGCAGCTTTGACACCAGAAATGGCGA
TTAAACTTTCCGTGGTGATCGGCAGTTCGCCGCAAATGTGGCTGAATCTGCAAAACGCCTGGAGTCTGGCAGAGGCAGAA
AAAACGGTGGATGTGTCGCGATTACGCCGTCTGGTTACGCAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829G6T1

Antitoxin

Source ID Structure
PDB 2ICT
PDB 2ICP
AlphaFold DB A0A829CUG6

References