Detailed information of TA system TA050451

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 86462..87064
• Replicon: chromosome
• Accession: NZ_CP010121
• Organism: Escherichia coli strain C4

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: RG27_RS00440
• Protein ID: WP_000897305.1
• Coordinates: 86753..87064 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: RG27_RS00435
• Protein ID: WP_000356395.1
• Coordinates: 86462..86752 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
RG27_RS00400	82085..82987	+	903	WP_000331377.1	formate dehydrogenase O subunit beta	-
RG27_RS00405	82984..83619	+	636	WP_000829010.1	formate dehydrogenase cytochrome b556 subunit	-
RG27_RS00410	83616..84545	+	930	WP_000027708.1	formate dehydrogenase accessory protein FdhE	-
RG27_RS00415	84727..84969	-	243	WP_001086386.1	ribbon-helix-helix domain-containing protein	-
RG27_RS00420	85188..85406	-	219	WP_001315113.1	ribbon-helix-helix domain-containing protein	-
RG27_RS00425	85825..86103	-	279	WP_001315112.1	hypothetical protein	-
RG27_RS00435	86462..86752	-	291	WP_000356395.1	helix-turn-helix domain-containing protein	Antitoxin
RG27_RS00440	86753..87064	-	312	WP_000897305.1	hypothetical protein	Toxin
RG27_RS00445	87293..88201	+	909	WP_001306651.1	alpha/beta hydrolase	-
RG27_RS00450	88370..90184	-	1815	WP_000226339.1	hypothetical protein	-
RG27_RS00455	90600..91541	-	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
RG27_RS00460	91586..92023	-	438	WP_000560983.1	D-tyrosyl-tRNA(Tyr) deacylase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T50451 WP_000897305.1 NZ_CP010121:c87064-86753 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T50451 NZ_CP010121:c87064-86753 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTGTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10869.46 Da
• Isoelectric Point: 10.8907

>AT50451 WP_000356395.1 NZ_CP010121:c86752-86462 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRHPSRTFEVDAMKIKEIRRASGLSQSKFAELISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT50451 NZ_CP010121:c86752-86462 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACACCCTTC
ACGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGAAC
TTATTTCCGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACTGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAGGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7