Detailed information of TA system TA050448

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 25475..25729
• Replicon: plasmid A
• Accession: NZ_CP010120
• Organism: Escherichia coli strain C3

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: -
• Locus tag: RG26_RS29195
• Protein ID: WP_032232098.1
• Coordinates: 25475..25681 (-)
• Length: 69 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	25668..25729 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
RG26_RS29145	20753..21028	-	276	WP_000421253.1	type II toxin-antitoxin system RelE/ParE family toxin	-
RG26_RS29150	21028..21312	-	285	WP_024182491.1	ribbon-helix-helix protein, CopG family	-
RG26_RS29160	22178..23035	-	858	WP_000774299.1	incFII family plasmid replication initiator RepA	-
RG26_RS29165	23028..23510	-	483	WP_000247130.1	hypothetical protein	-
RG26_RS29170	23503..23577	-	75	WP_032164225.1	RepA leader peptide Tap	-
RG26_RS29180	23812..24069	-	258	WP_000083826.1	replication regulatory protein RepA	-
RG26_RS29185	24378..24860	-	483	WP_000405244.1	GNAT family N-acetyltransferase	-
RG26_RS29190	24851..25153	-	303	WP_001346256.1	DUF1778 domain-containing protein	-
RG26_RS29195	25475..25681	-	207	WP_032232098.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	25668..25729	+	62	NuclAT_1	-	Antitoxin
-	25668..25729	+	62	NuclAT_1	-	Antitoxin
-	25668..25729	+	62	NuclAT_1	-	Antitoxin
-	25668..25729	+	62	NuclAT_1	-	Antitoxin
RG26_RS29205	26313..26525	-	213	WP_032323685.1	hypothetical protein	-
RG26_RS29210	26657..27217	-	561	WP_024244031.1	fertility inhibition protein FinO	-
RG26_RS29215	27272..28018	-	747	WP_000205747.1	conjugal transfer pilus acetylase TraX	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	faeH / faeF / faeE / faeD / faeC	1..63684	63684

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (23-64)

Sequences

Toxin

Protein

• Length: 69 a.a.
• Molecular weight: 7755.23 Da
• Isoelectric Point: 8.1204

>T50448 WP_032232098.1 NZ_CP010120:c25681-25475 [Escherichia coli]
MKYLNTTDCSLFFAERSKFMTKYALIGLLAVCATVLCFSLIFRELLCELNIHRGNTVVQVTLAYEAQK

Nucleotide

• Length: 207 bp

>T50448 NZ_CP010120:c25681-25475 [Escherichia coli]
ATGAAGTACCTTAACACTACTGATTGTAGCCTCTTCTTTGCAGAGAGGTCAAAGTTTATGACGAAATATGCCCTTATCGG
GTTGCTCGCCGTGTGCGCCACGGTGTTGTGTTTTTCACTGATATTCAGGGAACTGTTATGTGAGCTGAATATTCACAGAG
GAAATACAGTGGTGCAGGTAACTCTGGCCTACGAAGCACAGAAGTAA

Antitoxin

Nucleotide

• Length: 62 bp

>AT50448 NZ_CP010120:25668-25729 [Escherichia coli]
TTAAGGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Antitoxin