Detailed information of TA system TA050442

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 13006..13427
• Replicon: plasmid A
• Accession: NZ_CP010120
• Organism: Escherichia coli strain C3

Toxin (Protein)

• Gene name: hok
• Uniprot ID: B1VC78
• Locus tag: RG26_RS29065
• Protein ID: WP_001302184.1
• Coordinates: 13269..13427 (+)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	13006..13204 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
RG26_RS29015	8035..8226	+	192	WP_001027495.1	hypothetical protein	-
RG26_RS30890	8196..8705	-	510	WP_032163513.1	hypothetical protein	-
RG26_RS29035	9023..9475	+	453	WP_000290802.1	single-stranded DNA-binding protein	-
RG26_RS29040	9532..9765	+	234	WP_000005990.1	DUF905 family protein	-
RG26_RS29045	9831..11789	+	1959	WP_000117173.1	ParB/RepB/Spo0J family partition protein	-
RG26_RS29050	11844..12278	+	435	WP_000845928.1	conjugation system SOS inhibitor PsiB	-
RG26_RS29055	12275..12994	+	720	WP_000116348.1	plasmid SOS inhibition protein A	-
-	13006..13204	+	199	NuclAT_0	-	Antitoxin
-	13006..13204	+	199	NuclAT_0	-	Antitoxin
-	13006..13204	+	199	NuclAT_0	-	Antitoxin
-	13006..13204	+	199	NuclAT_0	-	Antitoxin
RG26_RS29065	13269..13427	+	159	WP_001302184.1	type I toxin-antitoxin system Hok family toxin	Toxin
RG26_RS30895	13665..14024	-	360	Protein_18	hypothetical protein	-
RG26_RS30900	14094..14300	+	207	WP_000547965.1	hypothetical protein	-
RG26_RS29080	14325..14612	+	288	WP_000107542.1	hypothetical protein	-
RG26_RS29085	14731..15552	+	822	WP_001234417.1	DUF945 domain-containing protein	-
RG26_RS29090	15848..16438	-	591	WP_001375132.1	transglycosylase SLT domain-containing protein	-
RG26_RS29100	16790..17173	+	384	WP_001063020.1	relaxosome protein TraM	-
RG26_RS29105	17341..18038	-	698	Protein_24	IS1-like element IS1A family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	faeH / faeF / faeE / faeD / faeC	1..63684	63684

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6108.35 Da
• Isoelectric Point: 9.1977

>T50442 WP_001302184.1 NZ_CP010120:13269-13427 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGYREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T50442 NZ_CP010120:13269-13427 [Escherichia coli]
ATGAAACTACCACGCAGCTCTCTTGTCTGGTGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGATACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 199 bp

>AT50442 NZ_CP010120:13006-13204 [Escherichia coli]
TCACACAGATTACCCGTAAACAGCCTGAATGAGCGGGTTATTTTCAGGAAAAGTGAGTGTGGTCAGCGTGCAGGGATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTGCCGTGTTGTGTGGCAGAAGGA
CAAAAGCCCCGTAGTTAATTTTTCATTAACCCACGAGGC

Structures

Antitoxin