Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 4221289..4221791 Replicon chromosome
Accession NZ_CP010119
Organism Escherichia coli strain C3

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag RG26_RS22460 Protein ID WP_000767829.1
Coordinates 4221537..4221791 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag RG26_RS22455 Protein ID WP_001259255.1
Coordinates 4221289..4221540 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
RG26_RS22425 4216467..4217534 - 1068 WP_000080093.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -
RG26_RS22430 4217534..4218604 - 1071 WP_001468894.1 histidinol-phosphate transaminase -
RG26_RS22435 4218601..4219905 - 1305 WP_021547561.1 histidinol dehydrogenase -
RG26_RS22440 4219911..4220810 - 900 WP_000131782.1 ATP phosphoribosyltransferase -
RG26_RS22450 4220956..4221006 - 51 WP_001364200.1 his operon leader peptide -
RG26_RS22455 4221289..4221540 + 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
RG26_RS22460 4221537..4221791 + 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
RG26_RS22465 4221874..4222698 + 825 WP_000754737.1 SDR family oxidoreductase -
RG26_RS22470 4222744..4223673 + 930 WP_000803360.1 LysR family transcriptional regulator -
RG26_RS30465 4223888..4223950 + 63 WP_010723108.1 membrane protein YoeI -
RG26_RS22475 4223940..4225298 + 1359 WP_000019197.1 putrescine/proton symporter PlaP -
RG26_RS22480 4225477..4226535 + 1059 WP_000492340.1 transport protein YeeE -
RG26_RS22485 4226549..4226776 + 228 WP_000985273.1 sulfurtransferase TusA family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T50432 WP_000767829.1 NZ_CP010119:4221537-4221791 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T50432 NZ_CP010119:4221537-4221791 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT50432 WP_001259255.1 NZ_CP010119:4221289-4221540 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT50432 NZ_CP010119:4221289-4221540 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References