Detailed information of TA system TA050415

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 1492347..1492949
• Replicon: chromosome
• Accession: NZ_CP010119
• Organism: Escherichia coli strain C3

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: RG26_RS07745
• Protein ID: WP_000897305.1
• Coordinates: 1492638..1492949 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: RG26_RS07740
• Protein ID: WP_000356397.1
• Coordinates: 1492347..1492637 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
RG26_RS07705	1487914..1488549	+	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
RG26_RS07710	1488546..1489475	+	930	WP_000027708.1	formate dehydrogenase accessory protein FdhE	-
RG26_RS07715	1489874..1490854	+	981	WP_000399648.1	IS110-like element IS621 family transposase	-
RG26_RS07725	1491082..1491324	-	243	WP_001086388.1	hypothetical protein	-
RG26_RS07730	1491543..1491761	-	219	WP_001295676.1	ribbon-helix-helix domain-containing protein	-
RG26_RS07740	1492347..1492637	-	291	WP_000356397.1	helix-turn-helix domain-containing protein	Antitoxin
RG26_RS07745	1492638..1492949	-	312	WP_000897305.1	hypothetical protein	Toxin
RG26_RS07750	1493178..1494086	+	909	WP_001371792.1	alpha/beta hydrolase	-
RG26_RS07755	1494150..1495091	-	942	WP_001300148.1	fatty acid biosynthesis protein FabY	-
RG26_RS07760	1495136..1495573	-	438	WP_000560983.1	D-tyrosyl-tRNA(Tyr) deacylase	-
RG26_RS07765	1495570..1496442	-	873	WP_000920762.1	virulence factor BrkB family protein	-
RG26_RS07770	1496436..1497035	-	600	WP_001295269.1	glucose-1-phosphatase	-
RG26_RS07775	1497134..1497919	-	786	WP_000059678.1	DeoR/GlpR family DNA-binding transcription regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	1489874..1490854	980

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T50415 WP_000897305.1 NZ_CP010119:c1492949-1492638 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T50415 NZ_CP010119:c1492949-1492638 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTTTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT50415 WP_000356397.1 NZ_CP010119:c1492637-1492347 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT50415 NZ_CP010119:c1492637-1492347 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
GCGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCTGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7