Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 2556020..2556561 Replicon chromosome
Accession NZ_CP010117
Organism Escherichia coli strain C2

Toxin (Protein)


Gene name yafQ Uniprot ID U9XPG0
Locus tag RG25_RS13300 Protein ID WP_000615976.1
Coordinates 2556283..2556561 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID S1EWQ0
Locus tag RG25_RS13295 Protein ID WP_000729704.1
Coordinates 2556020..2556280 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
RG25_RS13270 2551804..2552589 - 786 WP_000207568.1 putative lateral flagellar export/assembly protein LafU -
RG25_RS13275 2552561..2554273 + 1713 Protein_2431 flagellar biosynthesis protein FlhA -
RG25_RS13280 2554378..2554656 + 279 WP_000598760.1 type II toxin-antitoxin system HicA family toxin -
RG25_RS13285 2554649..2555005 + 357 WP_001030484.1 type II toxin-antitoxin system HicB family antitoxin -
RG25_RS13290 2555062..2555834 - 773 Protein_2434 C40 family peptidase -
RG25_RS13295 2556020..2556280 + 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
RG25_RS13300 2556283..2556561 + 279 WP_000615976.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
RG25_RS13305 2556717..2557457 + 741 WP_001225679.1 murein L,D-transpeptidase -
RG25_RS13310 2557428..2558195 - 768 WP_000333380.1 class II glutamine amidotransferase -
RG25_RS13320 2558401..2558979 - 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(1-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10800.59 Da        Isoelectric Point: 10.0702

>T50402 WP_000615976.1 NZ_CP010117:2556283-2556561 [Escherichia coli]
MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNALPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T50402 NZ_CP010117:2556283-2556561 [Escherichia coli]
ATGATTCAAAGGGATATTGAATACTCGGGACAATTTTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATGCTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9391.77 Da        Isoelectric Point: 5.0758

>AT50402 WP_000729704.1 NZ_CP010117:2556020-2556280 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT50402 NZ_CP010117:2556020-2556280 [Escherichia coli]
ATGGCTGCTAACGCATTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCGGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCGTTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB U9XPG0

Antitoxin

Source ID Structure
PDB 4ML0
AlphaFold DB A0A0E0Y6Z6

References