Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 3400642..3401144 Replicon chromosome
Accession NZ_CP010116
Organism Escherichia coli strain C1

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag RC72_RS17515 Protein ID WP_000767829.1
Coordinates 3400642..3400896 (-) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag RC72_RS17520 Protein ID WP_001259255.1
Coordinates 3400893..3401144 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
RC72_RS17490 3395657..3395884 - 228 WP_000985273.1 sulfurtransferase TusA family protein -
RC72_RS17495 3395898..3396956 - 1059 WP_000492315.1 transport protein YeeE -
RC72_RS17500 3397135..3398493 - 1359 WP_000019197.1 putrescine/proton symporter PlaP -
RC72_RS25780 3398483..3398545 - 63 WP_010723108.1 membrane protein YoeI -
RC72_RS17505 3398760..3399689 - 930 WP_000803351.1 LysR family transcriptional regulator -
RC72_RS17510 3399735..3400559 - 825 WP_000754749.1 SDR family oxidoreductase -
RC72_RS17515 3400642..3400896 - 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
RC72_RS17520 3400893..3401144 - 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
RC72_RS17525 3401427..3401477 + 51 WP_001364200.1 his operon leader peptide -
RC72_RS17535 3401623..3402522 + 900 WP_000131782.1 ATP phosphoribosyltransferase -
RC72_RS17540 3402528..3403832 + 1305 WP_042031741.1 histidinol dehydrogenase -
RC72_RS17545 3403829..3404899 + 1071 WP_001682999.1 histidinol-phosphate transaminase -
RC72_RS17550 3404899..3405966 + 1068 WP_001563933.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T50384 WP_000767829.1 NZ_CP010116:c3400896-3400642 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T50384 NZ_CP010116:c3400896-3400642 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT50384 WP_001259255.1 NZ_CP010116:c3401144-3400893 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT50384 NZ_CP010116:c3401144-3400893 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References