Detailed information of TA system TA050369

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): ralRA/-
• Location: 2667807..2668178
• Replicon: chromosome
• Accession: NZ_CP010116
• Organism: Escherichia coli strain C1

Toxin (Protein)

• Gene name: ralR
• Uniprot ID: F4VC37
• Locus tag: RC72_RS13555
• Protein ID: WP_001317028.1
• Coordinates: 2667984..2668178 (-)
• Length: 65 a.a.

Antitoxin (RNA)

Gene name	ralA
Locus tag	-
Coordinates	2667807..2667985 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
RC72_RS13530	2663762..2665135	+	1374	WP_001551034.1	ATP-dependent RNA helicase DbpA	-
RC72_RS13535	2665264..2666199	-	936	WP_001157407.1	tRNA 2-thiocytidine(32) synthetase TtcA	-
RC72_RS13540	2666251..2667486	-	1236	WP_072617251.1	site-specific integrase	-
RC72_RS13545	2667488..2667703	-	216	WP_000079604.1	excisionase XisR	-
RC72_RS13550	2667782..2667991	-	210	WP_000276809.1	double-strand break reduction protein RcbA	-
-	2667807..2667985	+	179	NuclAT_0	-	Antitoxin
-	2667807..2667985	+	179	NuclAT_0	-	Antitoxin
-	2667807..2667985	+	179	NuclAT_0	-	Antitoxin
-	2667807..2667985	+	179	NuclAT_0	-	Antitoxin
RC72_RS13555	2667984..2668178	-	195	WP_001317028.1	type I toxin-antitoxin system endodeoxyribonuclease toxin RalR	Toxin
RC72_RS13560	2668234..2669043	-	810	WP_000166319.1	recombination protein RecT	-
RC72_RS13565	2669036..2671636	-	2601	WP_072617252.1	exodeoxyribonuclease VIII	-
RC72_RS13570	2671736..2672011	-	276	WP_001372690.1	hypothetical protein	-
RC72_RS13575	2672086..2672256	-	171	WP_065336296.1	hypothetical protein	-
RC72_RS13580	2672256..2672477	-	222	WP_000560223.1	killing protein KilR	-
RC72_RS13585	2672899..2673051	-	153	WP_000379561.1	DUF1391 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	qnrS1	-	2666251..2721009	54758

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Endodeoxyribonuclease toxin RalR (6-54)

Sequences

Toxin

Protein

• Length: 65 a.a.
• Molecular weight: 7018.89 Da
• Isoelectric Point: 8.9538

>T50369 WP_001317028.1 NZ_CP010116:c2668178-2667984 [Escherichia coli]
MRYDNVKPCPFCGCPSVTVKAISGYYRAKCNGCESRTGYGGSEKEALERWNKRTTGNNNGGVHV

Nucleotide

• Length: 195 bp

>T50369 NZ_CP010116:c2668178-2667984 [Escherichia coli]
ATGAGATATGACAATGTTAAACCATGTCCATTTTGTGGTTGTCCATCAGTAACGGTGAAAGCCATTTCAGGATATTACCG
AGCGAAGTGTAACGGATGCGAATCCCGAACCGGTTATGGTGGAAGTGAAAAAGAAGCACTCGAAAGATGGAATAAACGAA
CCACTGGAAATAATAATGGAGGTGTTCATGTATAA

Antitoxin

Nucleotide

• Length: 179 bp

>AT50369 NZ_CP010116:2667807-2667985 [Escherichia coli]
GAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGA
GAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTT
CAATAGTGGCGGTAATTTT

Structures

Toxin

• AlphaFold DB: A0A7L6LHV7

Antitoxin