Detailed information of TA system TA050364

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 1588510..1589204
• Replicon: chromosome
• Accession: NZ_CP010116
• Organism: Escherichia coli strain C1

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: Q0T7Q5
• Locus tag: RC72_RS07980
• Protein ID: WP_001263491.1
• Coordinates: 1588806..1589204 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: L4JHF0
• Locus tag: RC72_RS07975
• Protein ID: WP_000554755.1
• Coordinates: 1588510..1588803 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
RC72_RS07950	1584131..1584487	-	357	WP_001030484.1	type II toxin-antitoxin system HicB family antitoxin	-
RC72_RS07955	1584480..1584758	-	279	WP_000598760.1	type II toxin-antitoxin system HicA family toxin	-
RC72_RS07960	1584863..1586575	-	1713	Protein_1424	flagellar biosynthesis protein FlhA	-
RC72_RS07965	1586547..1587332	+	786	WP_019842510.1	putative lateral flagellar export/assembly protein LafU	-
RC72_RS07970	1587403..1588458	+	1056	WP_001550655.1	DNA polymerase IV	-
RC72_RS07975	1588510..1588803	+	294	WP_000554755.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
RC72_RS07980	1588806..1589204	+	399	WP_001263491.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
RC72_RS07985	1589214..1589666	+	453	WP_019842500.1	GNAT family N-acetyltransferase	-
RC72_RS07990	1589912..1590118	+	207	Protein_1430	RtcB family protein	-
RC72_RS26095	1590114..1590466	+	353	Protein_1431	peptide chain release factor H	-
RC72_RS08000	1590523..1591980	-	1458	WP_001293009.1	cytosol nonspecific dipeptidase	-
RC72_RS08005	1592241..1592699	+	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
RC72_RS08010	1592791..1594035	+	1245	WP_000189541.1	esterase FrsA	-
-	1593295..1593375	+	81	NuclAT_11	-	-
-	1593295..1593375	+	81	NuclAT_11	-	-
-	1593295..1593375	+	81	NuclAT_11	-	-
-	1593295..1593375	+	81	NuclAT_11	-	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15457.86 Da
• Isoelectric Point: 8.0949

>T50364 WP_001263491.1 NZ_CP010116:1588806-1589204 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T50364 NZ_CP010116:1588806-1589204 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGTGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAGCCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGAAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11235.78 Da
• Isoelectric Point: 5.2170

>AT50364 WP_000554755.1 NZ_CP010116:1588510-1588803 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKLVKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT50364 NZ_CP010116:1588510-1588803 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAATCCAGCAAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CAGAACAAGAGGAGAAAAAACTGGTGAAGGCGCGCTTCCGTCCGAGTGCCGCAAGATTAGAGGAAATTACACGCCGCGCT
GAACAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Structures

Toxin

• AlphaFold DB: A0A4P7TPK7

Antitoxin

• AlphaFold DB: A0A829G9H5