Detailed information of TA system TA050359

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 693694..694296
• Replicon: chromosome
• Accession: NZ_CP010116
• Organism: Escherichia coli strain C1

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: RC72_RS03490
• Protein ID: WP_000897305.1
• Coordinates: 693694..694005 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: RC72_RS03495
• Protein ID: WP_000356395.1
• Coordinates: 694006..694296 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
RC72_RS03470	688736..689173	+	438	WP_000560983.1	D-tyrosyl-tRNA(Tyr) deacylase	-
RC72_RS03475	689218..690159	+	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
RC72_RS03480	690575..692389	+	1815	WP_001550352.1	hypothetical protein	-
RC72_RS03485	692557..693465	-	909	WP_001550353.1	alpha/beta hydrolase	-
RC72_RS03490	693694..694005	+	312	WP_000897305.1	hypothetical protein	Toxin
RC72_RS03495	694006..694296	+	291	WP_000356395.1	helix-turn-helix domain-containing protein	Antitoxin
RC72_RS03500	694381..694602	+	222	WP_001550354.1	hypothetical protein	-
RC72_RS03505	694654..694932	+	279	WP_001315112.1	hypothetical protein	-
RC72_RS03510	695351..695569	+	219	WP_001295676.1	ribbon-helix-helix domain-containing protein	-
RC72_RS03515	695788..696030	+	243	WP_001309881.1	ribbon-helix-helix domain-containing protein	-
RC72_RS03520	696212..697141	-	930	WP_000027720.1	formate dehydrogenase accessory protein FdhE	-
RC72_RS03525	697138..697773	-	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
RC72_RS03530	697770..698672	-	903	WP_000331377.1	formate dehydrogenase O subunit beta	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T50359 WP_000897305.1 NZ_CP010116:693694-694005 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T50359 NZ_CP010116:693694-694005 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTGTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10869.46 Da
• Isoelectric Point: 10.8907

>AT50359 WP_000356395.1 NZ_CP010116:694006-694296 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRHPSRTFEVDAMKIKEIRRASGLSQSKFAELISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT50359 NZ_CP010116:694006-694296 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACACCCTTC
ACGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGAAC
TTATTTCCGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACTGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAGGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7