Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 4694250..4694959 Replicon chromosome
Accession NZ_CP010114
Organism Mycobacterium avium subsp. paratuberculosis strain E93

Toxin (Protein)


Gene name HigB2 Uniprot ID X7V9P5
Locus tag RE97_RS21900 Protein ID WP_003873387.1
Coordinates 4694555..4694959 (-) Length 135 a.a.

Antitoxin (Protein)


Gene name HigA2 Uniprot ID Q73S10
Locus tag RE97_RS21895 Protein ID WP_003873388.1
Coordinates 4694250..4694558 (-) Length 103 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
RE97_RS21880 4690135..4690437 + 303 WP_003873391.1 co-chaperone GroES -
RE97_RS21885 4690526..4692142 + 1617 WP_003873390.1 chaperonin GroEL -
RE97_RS21890 4692237..4693763 + 1527 WP_003879517.1 adenylate/guanylate cyclase domain-containing protein -
RE97_RS21895 4694250..4694558 - 309 WP_003873388.1 helix-turn-helix transcriptional regulator Antitoxin
RE97_RS21900 4694555..4694959 - 405 WP_003873387.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
RE97_RS21905 4695199..4695891 + 693 WP_010950286.1 DUF4352 domain-containing protein -
RE97_RS21910 4695946..4697145 + 1200 WP_010950287.1 FAD-dependent oxidoreductase -
RE97_RS21915 4697302..4698066 - 765 WP_003874401.1 superoxide dismutase family protein -
RE97_RS21920 4698109..4698417 - 309 WP_003874400.1 WhiB family transcriptional regulator -
RE97_RS21925 4698851..4699629 + 779 Protein_4409 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(13-109)

Antitoxin

HigA2-like, helix-turn-helix domain

(31-91)


Sequences

Toxin        


Download         Length: 135 a.a.        Molecular weight: 15503.82 Da        Isoelectric Point: 10.3815

>T50358 WP_003873387.1 NZ_CP010114:c4694959-4694555 [Mycobacterium avium subsp. paratuberculosis]
VRKWEVDLTLIEAWMDALDDEEYDNLIAALEQLEEHGPITRRPFVDTLEGSRHPNMKELRPRPTKAGAHIRVLFAFDTRS
RAIMLIAGDKAGNWSKWYAKHIPIADELFDAHQKRLHKAAAKATNRKPRKGKKR

Download         Length: 405 bp

>T50358 NZ_CP010114:c4694959-4694555 [Mycobacterium avium subsp. paratuberculosis]
GTGCGGAAATGGGAGGTTGACCTGACTCTCATCGAAGCCTGGATGGATGCCCTCGATGACGAGGAGTACGACAACTTGAT
AGCAGCGCTCGAGCAGTTGGAGGAACACGGTCCAATTACCCGGCGGCCGTTTGTGGACACCCTTGAAGGGTCAAGGCACC
CGAACATGAAGGAGCTCCGTCCGCGCCCCACGAAAGCTGGAGCCCACATTCGCGTGCTATTCGCCTTCGATACCCGGTCG
CGGGCGATCATGTTGATCGCTGGAGACAAGGCGGGCAATTGGTCCAAGTGGTATGCCAAGCACATTCCCATTGCCGACGA
GCTCTTCGATGCTCACCAAAAGCGTTTACACAAGGCAGCAGCCAAAGCCACCAATCGGAAACCAAGGAAGGGAAAGAAAC
GATGA

Antitoxin


Download         Length: 103 a.a.        Molecular weight: 11445.94 Da        Isoelectric Point: 10.0801

>AT50358 WP_003873388.1 NZ_CP010114:c4694558-4694250 [Mycobacterium avium subsp. paratuberculosis]
MTNLDDMRRRRPGNRTRIDAIKAEMDREVAQYRLRELREAAGYTQTTLAAAIGVGQNRVSQMEHGDLGTSRVDTLRKYVE
ATGGELEVSVKRPDGSRVLLSL

Download         Length: 309 bp

>AT50358 NZ_CP010114:c4694558-4694250 [Mycobacterium avium subsp. paratuberculosis]
ATGACCAATCTTGATGACATGCGCCGTCGCCGTCCCGGCAACCGGACCCGTATCGACGCGATCAAGGCGGAGATGGACCG
CGAGGTTGCCCAGTACCGTCTCCGCGAACTGCGCGAGGCTGCCGGCTACACGCAGACGACTCTCGCTGCGGCTATTGGCG
TCGGGCAGAACCGCGTCTCGCAGATGGAACATGGCGATCTGGGCACGAGCCGGGTCGACACTCTCCGCAAGTACGTGGAA
GCGACTGGGGGTGAGCTGGAAGTGTCCGTCAAGCGTCCGGACGGGTCACGGGTTCTCCTCAGCCTGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB X7V9P5

Antitoxin

Source ID Structure
AlphaFold DB A0A7U4U5M0

References