Detailed information of TA system TA050272

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 2636773..2637526
• Replicon: chromosome
• Accession: NZ_CP010026
• Organism: Paraburkholderia fungorum strain ATCC BAA-463

Toxin (Protein)

• Gene name: graT
• Uniprot ID: A0A0D5V7W6
• Locus tag: OI25_RS17075
• Protein ID: WP_028200139.1
• Coordinates: 2637245..2637526 (-)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: OI25_RS17070
• Protein ID: WP_046568014.1
• Coordinates: 2636773..2637228 (-)
• Length: 152 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
OI25_RS43510	2632250..2632399	-	150	WP_028200145.1	hypothetical protein	-
OI25_RS17045	2632908..2634023	-	1116	WP_046568011.1	aldo/keto reductase	-
OI25_RS17050	2634146..2635063	+	918	WP_046570153.1	LysR family transcriptional regulator	-
OI25_RS17055	2635226..2635426	+	201	WP_028200142.1	hypothetical protein	-
OI25_RS17065	2635635..2636651	-	1017	WP_028200141.1	class 1 fructose-bisphosphatase	-
OI25_RS17070	2636773..2637228	-	456	WP_046568014.1	HigA family addiction module antidote protein	Antitoxin
OI25_RS17075	2637245..2637526	-	282	WP_028200139.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
OI25_RS17080	2637749..2640445	-	2697	WP_046568016.1	aminopeptidase N	-
OI25_RS17090	2640669..2641376	+	708	WP_028200136.1	DUF4136 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (23-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (35-84)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10547.19 Da
• Isoelectric Point: 9.0944

>T50272 WP_028200139.1 NZ_CP010026:c2637526-2637245 [Paraburkholderia fungorum]
MITTFGDKATAAIFHGKFVHSLPLYMQALARRKLLMIDAAESIRSLHAPPGNRLEALQGQRSGQWSIRINAQWRICFHFV
DGEALNVEIVDYH

Nucleotide

• Length: 282 bp

>T50272 NZ_CP010026:c2637526-2637245 [Paraburkholderia fungorum]
ATGATCACGACATTCGGCGACAAGGCTACGGCAGCAATCTTCCACGGCAAGTTCGTGCATTCACTGCCGCTCTACATGCA
GGCGCTCGCACGCCGCAAGCTGCTGATGATCGATGCCGCGGAGTCCATCCGCAGCCTGCATGCGCCGCCGGGCAACCGGC
TCGAAGCCTTGCAAGGCCAGCGCAGCGGGCAGTGGAGCATTCGCATCAACGCGCAGTGGCGCATCTGTTTTCATTTTGTC
GACGGGGAGGCGCTGAATGTCGAAATTGTCGACTATCACTGA

Antitoxin

Protein

• Length: 152 a.a.
• Molecular weight: 16369.88 Da
• Isoelectric Point: 10.3612

>AT50272 WP_046568014.1 NZ_CP010026:c2637228-2636773 [Paraburkholderia fungorum]
MVIKRSELGSIDFSDIGTGESIPETHPGEILRSEFLEPLGMSVNALALALRVPAPRINDIVRGKRAISADTALRLERYFG
ASAQFWLNLQIAYDLRVATAAAGEQIEREIEPMPRANRPKLPKVSAEHARAAALAASLTGNVTAIKARRKA

Nucleotide

• Length: 456 bp

>AT50272 NZ_CP010026:c2637228-2636773 [Paraburkholderia fungorum]
ATGGTCATCAAACGCTCCGAACTGGGCAGTATCGACTTCTCCGACATCGGCACGGGAGAAAGCATCCCGGAGACTCACCC
GGGGGAGATTCTGCGCAGCGAGTTCCTCGAGCCGCTCGGCATGTCCGTCAATGCGCTCGCACTTGCGCTGCGAGTACCGG
CGCCGCGCATCAACGACATCGTGCGCGGCAAACGCGCCATCTCGGCTGACACCGCACTGCGGCTCGAACGCTACTTTGGC
GCGAGTGCGCAGTTCTGGCTAAATCTGCAGATCGCTTACGACCTGCGCGTCGCCACCGCCGCGGCCGGCGAGCAGATCGA
ACGCGAGATCGAGCCGATGCCCAGGGCGAACCGGCCGAAACTGCCGAAAGTCTCGGCCGAACACGCCCGGGCGGCGGCGC
TTGCGGCGAGCCTCACAGGCAATGTCACGGCCATCAAGGCTCGCCGCAAAGCGTGA

Structures

Toxin

• AlphaFold DB: A0A0D5V7W6