Detailed information of TA system TA050197

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mqsRA (relBE)/MqsR-MqsA
• Location: 259543..260243
• Replicon: chromosome
• Accession: NZ_CP009974
• Organism: Pseudomonas putida S12

Toxin (Protein)

• Gene name: mqsR
• Uniprot ID: -
• Locus tag: RPPX_RS01150
• Protein ID: WP_023389615.1
• Coordinates: 259947..260243 (-)
• Length: 99 a.a.

Antitoxin (Protein)

• Gene name: mqsA
• Uniprot ID: A0A2L1I9E4
• Locus tag: RPPX_RS01145
• Protein ID: WP_019437852.1
• Coordinates: 259543..259944 (-)
• Length: 134 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
RPPX_RS01125	254625..255446	-	822	WP_019437851.1	transporter substrate-binding domain-containing protein	-
RPPX_RS01130	255522..256451	-	930	WP_003254230.1	FAD-binding protein	-
RPPX_RS01135	256452..257201	-	750	WP_014590265.1	electron transfer flavoprotein subunit beta/FixA family protein	-
RPPX_RS01140	257737..259419	+	1683	WP_023389616.1	electron transfer flavoprotein-ubiquinone oxidoreductase	-
RPPX_RS01145	259543..259944	-	402	WP_019437852.1	type II toxin-antitoxin system MqsA family antitoxin	Antitoxin
RPPX_RS01150	259947..260243	-	297	WP_023389615.1	type II toxin-antitoxin system MqsR family toxin	Toxin
RPPX_RS01155	260338..261663	-	1326	WP_019437854.1	MFS transporter	-
RPPX_RS01160	261736..262215	-	480	WP_019437855.1	hypothetical protein	-
RPPX_RS01165	262384..262860	-	477	WP_019437856.1	sigma-70 family RNA polymerase sigma factor	-
RPPX_RS01170	263073..264251	+	1179	WP_014590258.1	efflux RND transporter periplasmic adaptor subunit	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin MqsR (2-97)

Antitoxin

• Antitoxin MqsA/HigA-2 (1-131)

Sequences

Toxin

Protein

• Length: 99 a.a.
• Molecular weight: 11165.97 Da
• Isoelectric Point: 8.8385

>T50197 WP_023389615.1 NZ_CP009974:c260243-259947 [Pseudomonas putida S12]
MEKRTPHCPLERVRALVGAGRISPTTASLRGAQALGMEYADMLEVITGLERRDFYKSMTSYADHRVWQDVYRPLTVKGYL
YLKLSVVDDALIVSFKEL

Nucleotide

• Length: 297 bp

>T50197 NZ_CP009974:c260243-259947 [Pseudomonas putida S12]
ATGGAAAAGAGAACGCCTCATTGCCCGCTGGAGCGGGTAAGAGCACTGGTGGGCGCTGGCCGCATCAGCCCTACAACCGC
TTCCTTACGAGGCGCTCAGGCCTTGGGAATGGAATATGCGGACATGCTGGAGGTCATCACCGGTCTGGAGCGCAGAGATT
TCTACAAAAGCATGACCAGTTATGCCGACCACCGCGTTTGGCAAGATGTCTATCGGCCGCTTACGGTAAAGGGATATCTG
TACCTGAAACTGTCAGTGGTAGATGACGCGCTCATCGTGTCTTTCAAGGAGTTGTGA

Antitoxin

Protein

• Length: 134 a.a.
• Molecular weight: 14818.86 Da
• Isoelectric Point: 4.6580

>AT50197 WP_019437852.1 NZ_CP009974:c259944-259543 [Pseudomonas putida S12]
MRCPICGGAELALDTQDMPYRYKGESTLIPDVTGDYCSVCGEAVLSHDEAMRVSDLMSAFNRQVNALDVDPDFIVSVRKK
FDLDQREAGEIFGGGVNAFSRYENGKTKPPVALIKLFKLLDRHPELFEEVRTA

Nucleotide

• Length: 402 bp

>AT50197 NZ_CP009974:c259944-259543 [Pseudomonas putida S12]
ATGAGATGCCCAATTTGCGGAGGTGCCGAGCTCGCGCTAGACACTCAGGACATGCCCTACCGGTATAAGGGGGAATCGAC
CCTGATACCTGATGTGACGGGCGATTACTGTTCTGTCTGTGGTGAGGCAGTGCTAAGTCATGACGAGGCTATGCGAGTCA
GCGATCTGATGTCTGCCTTCAATCGCCAAGTCAATGCTCTAGACGTTGATCCCGATTTCATCGTTTCCGTACGCAAGAAA
TTTGATCTGGATCAGCGGGAGGCTGGGGAAATATTCGGTGGAGGAGTCAATGCTTTTTCACGTTATGAGAATGGCAAAAC
CAAACCGCCGGTGGCGCTGATCAAATTGTTCAAGTTGCTCGACCGTCACCCGGAGCTCTTTGAGGAAGTCAGGACTGCGT
GA

Structures

Antitoxin

• AlphaFold DB: A0A2L1I9E4