Detailed information of TA system TA050172

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 1811..2451
• Replicon: plasmid pPCP
• Accession: NZ_CP009936
• Organism: Yersinia pestis Angola

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: CH56_RS21825
• Protein ID: WP_002225538.1
• Coordinates: 2110..2451 (-)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: Q9S1D9
• Locus tag: CH56_RS21820
• Protein ID: WP_002213446.1
• Coordinates: 1811..2110 (-)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CH56_RS21810	138..443	+	306	WP_002221218.1	hypothetical protein	-
CH56_RS21815	686..1624	+	939	WP_002231174.1	omptin family plasminogen activator Pla	-
CH56_RS21820	1811..2110	-	300	WP_002213446.1	XRE family transcriptional regulator	Antitoxin
CH56_RS21825	2110..2451	-	342	WP_002225538.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
CH56_RS24300	3718..5193	+	1476	WP_012229282.1	IS21 family transposase	-
CH56_RS21840	6230..6424	+	195	WP_002213433.1	Rop family plasmid primer RNA-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	pla	1..9282	9282

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (10-103)

Antitoxin

• Cro/C1-type helix-turn-helix domain (32-80)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 13288.22 Da
• Isoelectric Point: 8.8337

>T50172 WP_002225538.1 NZ_CP009936:c2451-2110 [Yersinia pestis Angola]
MVLFSQRFDDWLNEQEDALQEKVLADLKKLQVYGPELPRPYADTVKGSRYKNMKELRVQFSGRPIRAFYAFDPIRRAIVL
CAGDKSNDKRFYEKLVRIAEDEFTAHLNTLESK

Nucleotide

• Length: 342 bp

>T50172 NZ_CP009936:c2451-2110 [Yersinia pestis Angola]
ATGGTACTTTTTAGCCAGCGATTTGACGACTGGCTAAATGAGCAAGAAGACGCTCTTCAGGAAAAGGTTTTGGCTGATTT
GAAAAAGCTACAGGTTTACGGACCTGAGTTACCCAGGCCATATGCCGATACCGTGAAAGGTTCTCGGTACAAAAATATGA
AAGAGCTTCGCGTTCAGTTTTCTGGCCGTCCGATAAGAGCCTTTTATGCGTTCGATCCGATTCGTCGGGCTATCGTTCTT
TGTGCAGGAGATAAAAGTAATGATAAGCGGTTTTATGAAAAACTGGTGCGTATAGCTGAGGATGAATTTACAGCACATCT
GAACACACTGGAGAGCAAGTAA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 11127.94 Da
• Isoelectric Point: 5.8900

>AT50172 WP_002213446.1 NZ_CP009936:c2110-1811 [Yersinia pestis Angola]
MRTLDEVIASRSPESQTRIKEMADEMILEVGLQMMREELQLSQKQVAEAMGISQPAVTKLEQRGNDLKLATLKRYVEAMG
GKLSLDVELPTGRRVAFHV

Nucleotide

• Length: 300 bp

>AT50172 NZ_CP009936:c2110-1811 [Yersinia pestis Angola]
ATGAGAACATTAGATGAGGTGATTGCCAGTCGTTCACCTGAAAGCCAGACACGAATTAAAGAAATGGCAGATGAGATGAT
TCTTGAGGTCGGCTTGCAGATGATGCGTGAAGAACTCCAGTTATCACAAAAACAAGTTGCTGAGGCGATGGGTATAAGCC
AGCCAGCAGTAACAAAGCTGGAGCAGCGCGGAAATGATTTAAAGCTGGCGACGTTAAAGCGTTACGTTGAAGCAATGGGA
GGCAAATTAAGCTTGGATGTTGAGCTTCCTACAGGAAGGAGAGTAGCGTTCCATGTCTAA

Structures

Antitoxin

• AlphaFold DB: A0A080J016