Detailed information of TA system TA050112

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 851573..852140
• Replicon: chromosome
• Accession: NZ_CP009885
• Organism: Xylella fastidiosa strain Hib4

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A1L2B3Z4
• Locus tag: XFHB_RS04050
• Protein ID: WP_042463596.1
• Coordinates: 851859..852140 (-)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: graA
• Uniprot ID: -
• Locus tag: XFHB_RS04045
• Protein ID: WP_010893244.1
• Coordinates: 851573..851848 (-)
• Length: 92 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
XFHB_RS04015	847467..849347	+	1881	WP_088577836.1	phage major capsid protein	-
XFHB_RS04020	849365..849625	+	261	WP_060872391.1	hypothetical protein	-
XFHB_RS04025	849625..849948	+	324	WP_088577837.1	hypothetical protein	-
XFHB_RS04030	849945..850466	+	522	WP_088577838.1	hypothetical protein	-
XFHB_RS04035	850442..850984	+	543	WP_088577839.1	hypothetical protein	-
XFHB_RS04040	850981..851568	+	588	WP_088577840.1	phage baseplate assembly protein V	-
XFHB_RS04045	851573..851848	-	276	WP_010893244.1	HigA family addiction module antidote protein	Antitoxin
XFHB_RS04050	851859..852140	-	282	WP_042463596.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
XFHB_RS04055	852197..852496	-	300	WP_088577841.1	HigA family addiction module antidote protein	-
XFHB_RS04060	852500..852778	-	279	WP_088577842.1	type II toxin-antitoxin system RelE/ParE family toxin	-
XFHB_RS04065	852869..853207	+	339	WP_042462908.1	GPW/gp25 family protein	-
XFHB_RS04070	853207..854100	+	894	WP_088577843.1	baseplate J/gp47 family protein	-
XFHB_RS04075	854093..854650	+	558	WP_088577844.1	phage tail protein I	-
XFHB_RS04080	854658..856250	+	1593	WP_088577845.1	tail fiber domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	801718..868811	67093

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (2-93)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (1-88)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10578.06 Da
• Isoelectric Point: 9.8536

>T50112 WP_042463596.1 NZ_CP009885:c852140-851859 [Xylella fastidiosa]
VIKSFRHKGIQQFFLEGSTAGIQTKHAAKLRIQLTALESAKHPKDMNAPGWKLHSLKGADLKGHWSIWVNGNYRLTFAFE
GEDAILVDYQDYH

Nucleotide

• Length: 282 bp

>T50112 NZ_CP009885:c852140-851859 [Xylella fastidiosa]
GTGATCAAAAGTTTCCGCCACAAAGGCATTCAACAATTCTTCCTCGAGGGATCAACCGCTGGTATCCAAACCAAACACGC
CGCCAAACTGCGTATCCAGCTCACCGCGTTGGAGAGTGCTAAGCACCCCAAAGATATGAATGCTCCAGGCTGGAAACTGC
ACTCACTAAAAGGCGCTGACTTAAAAGGTCATTGGTCCATCTGGGTAAATGGCAACTATCGCCTCACGTTTGCTTTTGAA
GGCGAAGACGCAATCTTGGTCGACTATCAGGATTACCACTAG

Antitoxin

Protein

• Length: 92 a.a.
• Molecular weight: 10056.68 Da
• Isoelectric Point: 10.2184

>AT50112 WP_010893244.1 NZ_CP009885:c851848-851573 [Xylella fastidiosa]
MKRMHNPAHPGEVLREYLGDLTVTNAAAKLGVSRVALSRILNGANGISADMALRLEDALGTSAEMWVAMQFKYDLWVASQ
QPRPKITRLHV

Nucleotide

• Length: 276 bp

>AT50112 NZ_CP009885:c851848-851573 [Xylella fastidiosa]
ATGAAACGTATGCACAATCCCGCACATCCAGGTGAAGTGTTGCGTGAGTATCTTGGTGATCTCACTGTGACCAATGCGGC
TGCAAAGCTTGGCGTCAGCCGTGTCGCTTTGTCGCGTATTCTCAATGGTGCCAACGGCATCTCTGCCGATATGGCCCTAC
GCCTGGAAGATGCGCTTGGCACCAGTGCAGAGATGTGGGTAGCCATGCAGTTCAAGTACGACCTGTGGGTTGCCAGTCAG
CAGCCACGCCCGAAGATCACACGCCTTCATGTGTAA

Structures

Toxin

• AlphaFold DB: A0A1L2B3Z4