Detailed information of TA system TA050050

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 254..900
• Replicon: plasmid pECO-b75
• Accession: NZ_CP009861
• Organism: Escherichia coli strain ECONIH1

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: ECONIH1_RS27150
• Protein ID: WP_000269912.1
• Coordinates: 254..601 (+)
• Length: 116 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: ECONIH1_RS27155
• Protein ID: WP_001259435.1
• Coordinates: 601..900 (+)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ECONIH1_RS27150	254..601	+	348	WP_000269912.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
ECONIH1_RS27155	601..900	+	300	WP_001259435.1	XRE family transcriptional regulator	Antitoxin
ECONIH1_RS27160	1064..1444	+	381	WP_001523084.1	hypothetical protein	-
ECONIH1_RS27165	1548..1973	-	426	WP_016240784.1	hypothetical protein	-
ECONIH1_RS27170	2075..2335	+	261	WP_001523087.1	helix-turn-helix domain-containing protein	-
ECONIH1_RS27175	2958..3146	+	189	WP_033545979.1	hypothetical protein	-
ECONIH1_RS27180	3157..4326	-	1170	WP_033545977.1	ORF6N domain-containing protein	-
ECONIH1_RS27185	4323..4514	-	192	WP_000183351.1	hypothetical protein	-
ECONIH1_RS27190	5459..5707	-	249	WP_001523089.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..47560	47560

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-105)

Antitoxin

• Cro/C1-type helix-turn-helix domain (32-80)

Sequences

Toxin

Protein

• Length: 116 a.a.
• Molecular weight: 13545.51 Da
• Isoelectric Point: 8.8337

>T50050 WP_000269912.1 NZ_CP009861:254-601 [Escherichia coli]
MWTVLFSQRFDDWLNEQEDALQEKVLADLKKLQVYGPELPRPYADTVKGSRYKNMKELRVQFSGRPIRAFYAFDPIRRAI
VLCAGDKSNDKRFYEKLVRIAEDEFAAHLNTLESK

Nucleotide

• Length: 348 bp

>T50050 NZ_CP009861:254-601 [Escherichia coli]
ATGTGGACGGTACTTTTTAGCCAGCGATTTGACGACTGGCTAAATGAGCAAGAAGACGCTCTTCAGGAAAAGGTTTTGGC
TGATTTGAAAAAGCTACAGGTTTACGGACCTGAGTTACCCAGGCCATATGCCGATACCGTAAAAGGTTCTCGGTACAAAA
ATATGAAAGAGCTTCGCGTTCAGTTTTCTGGCCGTCCGATAAGAGCCTTTTATGCGTTCGATCCGATTCGCCGGGCCATC
GTTCTTTGTGCAGGAGACAAAAGTAACGATAAGCGGTTTTATGAAAAACTGGTGCGTATAGCTGAGGATGAGTTTGCAGC
ACATCTGAACACACTGGAGAGCAAGTAA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 11046.87 Da
• Isoelectric Point: 5.2090

>AT50050 WP_001259435.1 NZ_CP009861:601-900 [Escherichia coli]
MRTLDEAIASRSPESQARIKEMADEMILEVGLQMMREELQLSQKQVAEAMGISQPAVTKLEQRGNDLKLATLKRYVEAMG
GKLSLDVELPTGKRIAFNI

Nucleotide

• Length: 300 bp

>AT50050 NZ_CP009861:601-900 [Escherichia coli]
ATGAGAACATTAGATGAGGCGATTGCCAGCCGTTCACCTGAAAGCCAGGCGCGAATTAAAGAAATGGCAGATGAGATGAT
TCTTGAGGTCGGCTTGCAGATGATGCGTGAAGAACTCCAGTTATCACAAAAACAGGTTGCTGAGGCGATGGGTATAAGCC
AGCCAGCAGTAACAAAGCTGGAGCAGCGCGGAAATGATTTAAAGCTGGCGACATTAAAGCGTTACGTTGAAGCTATGGGA
GGCAAATTAAGCCTGGATGTTGAGCTTCCCACAGGAAAACGAATAGCGTTTAACATCTGA