Detailed information of TA system TA050046

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 105091..105420
• Replicon: plasmid pECO-824
• Accession: NZ_CP009860
• Organism: Escherichia coli strain ECONIH1

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: ECONIH1_RS27020
• Protein ID: WP_001312861.1
• Coordinates: 105091..105249 (-)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	105293..105420 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ECONIH1_RS26980	100203..100430	-	228	WP_001254388.1	conjugal transfer relaxosome protein TraY	-
ECONIH1_RS26985	100518..101195	-	678	WP_001348626.1	PAS domain-containing protein	-
ECONIH1_RS26990	101329..101712	-	384	WP_001151566.1	relaxosome protein TraM	-
ECONIH1_RS26995	102043..102645	+	603	WP_000243712.1	transglycosylase SLT domain-containing protein	-
ECONIH1_RS27000	102942..103763	-	822	WP_001234469.1	DUF945 domain-containing protein	-
ECONIH1_RS27005	103882..104169	-	288	WP_000107535.1	hypothetical protein	-
ECONIH1_RS30915	104194..104400	-	207	WP_000275859.1	hypothetical protein	-
ECONIH1_RS32085	104313..104648	-	336	WP_013023876.1	hypothetical protein	-
ECONIH1_RS27020	105091..105249	-	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	105293..105420	-	128	NuclAT_0	-	Antitoxin
-	105293..105420	-	128	NuclAT_0	-	Antitoxin
-	105293..105420	-	128	NuclAT_0	-	Antitoxin
-	105293..105420	-	128	NuclAT_0	-	Antitoxin
-	106862..106964	-	103	NuclAT_1	-	-
-	106862..106964	-	103	NuclAT_1	-	-
-	106862..106964	-	103	NuclAT_1	-	-
-	106862..106964	-	103	NuclAT_1	-	-
ECONIH1_RS27040	106976..107695	-	720	WP_001276232.1	plasmid SOS inhibition protein A	-
ECONIH1_RS27045	107692..108126	-	435	WP_000845940.1	conjugation system SOS inhibitor PsiB	-
ECONIH1_RS27050	108181..110139	-	1959	WP_000117179.1	ParB/RepB/Spo0J family partition protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	sitABCD / erm(B) / mph(A) / sul1 / qacE / aadA5 / blaCTX-M-15	iucA / iucB / iucC / iucD / iutA	1..121385	121385

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T50046 WP_001312861.1 NZ_CP009860:c105249-105091 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T50046 NZ_CP009860:c105249-105091 [Escherichia coli]
ATGAAACTACCACGAAGTTCCCTTGTCTGGTGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGACACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 128 bp

>AT50046 NZ_CP009860:c105420-105293 [Escherichia coli]
CGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTGCCGTGTTGTGTGGCAGAAAGAAGATAGCCCCGTAGTAA
GTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin