Detailed information of TA system TA050042

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 80760..80999
• Replicon: plasmid pECO-824
• Accession: NZ_CP009860
• Organism: Escherichia coli strain ECONIH1

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: A0A762TWR7
• Locus tag: ECONIH1_RS26890
• Protein ID: WP_023144756.1
• Coordinates: 80760..80894 (-)
• Length: 45 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	80939..80999 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ECONIH1_RS26865	77089..77946	-	858	WP_000130640.1	incFII family plasmid replication initiator RepA	-
ECONIH1_RS26870	77939..78013	-	75	WP_031943482.1	RepA leader peptide Tap	-
ECONIH1_RS32080	78010..78144	-	135	Protein_90	protein CopA/IncA	-
ECONIH1_RS30075	78250..78402	-	153	WP_072258247.1	replication protein A	-
ECONIH1_RS26880	78483..79505	+	1023	WP_000255944.1	IS21-like element IS100 family transposase	-
ECONIH1_RS26885	79502..80284	+	783	WP_001300609.1	IS21-like element IS100kyp family helper ATPase IstB	-
ECONIH1_RS30080	80353..80463	-	111	Protein_94	replication protein	-
ECONIH1_RS26890	80760..80894	-	135	WP_023144756.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	80939..80999	+	61	NuclAT_2	-	Antitoxin
-	80939..80999	+	61	NuclAT_2	-	Antitoxin
-	80939..80999	+	61	NuclAT_2	-	Antitoxin
-	80939..80999	+	61	NuclAT_2	-	Antitoxin
ECONIH1_RS26895	80966..81252	-	287	Protein_96	DUF2726 domain-containing protein	-
ECONIH1_RS26900	81765..81977	-	213	WP_013023861.1	hypothetical protein	-
ECONIH1_RS26905	82108..82668	-	561	WP_000139341.1	fertility inhibition protein FinO	-
ECONIH1_RS26910	82723..83469	-	747	WP_000205718.1	conjugal transfer pilus acetylase TraX	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	sitABCD / erm(B) / mph(A) / sul1 / qacE / aadA5 / blaCTX-M-15	iucA / iucB / iucC / iucD / iutA	1..121385	121385

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (4-44)

Sequences

Toxin

Protein

• Length: 45 a.a.
• Molecular weight: 4896.90 Da
• Isoelectric Point: 7.7209

>T50042 WP_023144756.1 NZ_CP009860:c80894-80760 [Escherichia coli]
MTKYTLIGLLAVCATVLCFSLIFREQLCELNIHRGNTVVQVTLA

Nucleotide

• Length: 135 bp

>T50042 NZ_CP009860:c80894-80760 [Escherichia coli]
ATGACGAAATATACCCTTATCGGGTTGCTTGCCGTGTGCGCCACGGTGTTGTGTTTTTCACTGATATTCAGGGAACAGTT
ATGTGAACTGAATATTCACAGGGGAAATACAGTGGTGCAGGTAACTCTGGCCTGA

Antitoxin

Nucleotide

• Length: 61 bp

>AT50042 NZ_CP009860:80939-80999 [Escherichia coli]
TAAAGTGCATCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Toxin

• AlphaFold DB: A0A762TWR7

Antitoxin