Detailed information of TA system TA050030

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4617795..4618397
• Replicon: chromosome
• Accession: NZ_CP009859
• Organism: Escherichia coli strain ECONIH1

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: ECONIH1_RS22895
• Protein ID: WP_000897305.1
• Coordinates: 4617795..4618106 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: ECONIH1_RS22900
• Protein ID: WP_000356397.1
• Coordinates: 4618107..4618397 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ECONIH1_RS22865	4612825..4613610	+	786	WP_000059679.1	DeoR/GlpR family DNA-binding transcription regulator	-
ECONIH1_RS22870	4613709..4614308	+	600	WP_001314324.1	glucose-1-phosphatase	-
ECONIH1_RS22875	4614302..4615174	+	873	WP_000920762.1	virulence factor BrkB family protein	-
ECONIH1_RS22880	4615171..4615608	+	438	WP_000560983.1	D-tyrosyl-tRNA(Tyr) deacylase	-
ECONIH1_RS22885	4615653..4616594	+	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
ECONIH1_RS22890	4616658..4617566	-	909	WP_001318161.1	alpha/beta hydrolase	-
ECONIH1_RS22895	4617795..4618106	+	312	WP_000897305.1	hypothetical protein	Toxin
ECONIH1_RS22900	4618107..4618397	+	291	WP_000356397.1	helix-turn-helix domain-containing protein	Antitoxin
ECONIH1_RS22910	4619002..4619220	+	219	WP_001298592.1	ribbon-helix-helix domain-containing protein	-
ECONIH1_RS22915	4619435..4620364	-	930	WP_000027720.1	formate dehydrogenase accessory protein FdhE	-
ECONIH1_RS22920	4620361..4620996	-	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
ECONIH1_RS22925	4620993..4621895	-	903	WP_000331377.1	formate dehydrogenase O subunit beta	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T50030 WP_000897305.1 NZ_CP009859:4617795-4618106 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T50030 NZ_CP009859:4617795-4618106 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTGTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT50030 WP_000356397.1 NZ_CP009859:4618107-4618397 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT50030 NZ_CP009859:4618107-4618397 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
GCGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCTGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7