Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 282443..283137 Replicon chromosome
Accession NZ_CP009859
Organism Escherichia coli strain ECONIH1

Toxin (Protein)


Gene name yafO Uniprot ID Q0T7Q5
Locus tag ECONIH1_RS01325 Protein ID WP_001263491.1
Coordinates 282739..283137 (+) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID L4JHF0
Locus tag ECONIH1_RS01320 Protein ID WP_000554755.1
Coordinates 282443..282736 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ECONIH1_RS01295 277479..277739 - 261 WP_000729708.1 type II toxin-antitoxin system antitoxin DinJ -
ECONIH1_RS01300 277925..278698 + 774 WP_022645219.1 C40 family peptidase -
ECONIH1_RS01305 278796..280508 - 1713 Protein_248 flagellar biosynthesis protein FlhA -
ECONIH1_RS01310 280480..281265 + 786 WP_072224360.1 putative lateral flagellar export/assembly protein LafU -
ECONIH1_RS01315 281336..282391 + 1056 WP_022645222.1 DNA polymerase IV -
ECONIH1_RS01320 282443..282736 + 294 WP_000554755.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
ECONIH1_RS01325 282739..283137 + 399 WP_001263491.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
ECONIH1_RS01330 283147..283599 + 453 WP_023909048.1 GNAT family N-acetyltransferase -
ECONIH1_RS01335 283790..284929 + 1140 WP_022645224.1 RNA ligase RtcB family protein -
ECONIH1_RS01340 284926..285540 + 615 WP_022645225.1 peptide chain release factor H -
ECONIH1_RS01345 285602..286111 + 510 WP_001361775.1 hydrolase -
ECONIH1_RS01350 286128..286409 - 282 WP_022645226.1 hypothetical protein -
ECONIH1_RS01355 286418..287875 - 1458 WP_022645227.1 cytosol nonspecific dipeptidase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15457.86 Da        Isoelectric Point: 8.0949

>T50007 WP_001263491.1 NZ_CP009859:282739-283137 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

>T50007 NZ_CP009859:282739-283137 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACAGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGTGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11235.78 Da        Isoelectric Point: 5.2170

>AT50007 WP_000554755.1 NZ_CP009859:282443-282736 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKLVKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Download         Length: 294 bp

>AT50007 NZ_CP009859:282443-282736 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTTGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAACTGGTTAAGGCGCGCTTCCGTCCGAGTGCCGCAAGATTAGAGGAAATTACACGCCGCGCT
GAACAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4P7TPK7

Antitoxin

Source ID Structure
AlphaFold DB A0A829G9H5

References