Detailed information of TA system TA049990

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 141220..141866
• Replicon: chromosome
• Accession: NZ_CP009854
• Organism: UNVERIFIED_ORG: Enterobacter cloacae strain ECNIH5

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: ECNIH5_RS00680
• Protein ID: WP_038415013.1
• Coordinates: 141516..141866 (-)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: ECNIH5_RS00675
• Protein ID: WP_038415012.1
• Coordinates: 141220..141519 (-)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ECNIH5_RS00650 (ECNIH5_00650)	136688..137272	-	585	WP_038415008.1	fimbrial protein	-
ECNIH5_RS00655 (ECNIH5_00655)	137325..137927	-	603	WP_038415009.1	LuxR C-terminal-related transcriptional regulator	-
ECNIH5_RS00660 (ECNIH5_00660)	138762..139550	+	789	WP_038415010.1	winged helix-turn-helix domain-containing protein	-
ECNIH5_RS00665 (ECNIH5_00665)	139552..140031	+	480	WP_022646606.1	hypothetical protein	-
ECNIH5_RS00670 (ECNIH5_00670)	140166..141155	+	990	WP_227022512.1	helix-turn-helix domain-containing protein	-
ECNIH5_RS00675 (ECNIH5_00675)	141220..141519	-	300	WP_038415012.1	XRE family transcriptional regulator	Antitoxin
ECNIH5_RS00680 (ECNIH5_00680)	141516..141866	-	351	WP_038415013.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
ECNIH5_RS24775 (ECNIH5_00685)	142102..142386	-	285	WP_227022513.1	DNA-binding protein	-
ECNIH5_RS00690 (ECNIH5_00690)	142489..142830	-	342	WP_038415015.1	SymE family type I addiction module toxin	-
ECNIH5_RS00695 (ECNIH5_00695)	142956..143147	+	192	WP_038416963.1	toxin-antitoxin system HicB family antitoxin	-
ECNIH5_RS00700 (ECNIH5_00700)	143153..143779	-	627	WP_051979808.1	hypothetical protein	-
ECNIH5_RS00710 (ECNIH5_00710)	144243..145634	+	1392	WP_023299010.1	glycoside-pentoside-hexuronide family transporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-105)

Antitoxin

• HigA2-like, helix-turn-helix domain (12-88)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 13521.57 Da
• Isoelectric Point: 8.8448

>T49990 WP_038415013.1 NZ_CP009854:c141866-141516 [Enterobacter cloacae]
MWTVLFSKVFEQWLLEQEDGLQEKVLADLLNLQNYGPRLPRPYADTVKGSQYKNMKELRIQYAGRPVRAFFAFDPVRQAI
VLCAGDKRNDKTFYEKLIRIADAEFTHHLTSLEAAK

Nucleotide

• Length: 351 bp

>T49990 NZ_CP009854:c141866-141516 [Enterobacter cloacae]
ATGTGGACGGTACTGTTTAGTAAGGTATTCGAGCAATGGTTGCTTGAACAGGAAGATGGGTTGCAGGAAAAGGTACTGGC
AGACCTGTTAAATCTACAGAATTATGGACCGCGTCTTCCTCGTCCCTATGCGGATACCGTTAAGGGCTCACAGTATAAGA
ATATGAAAGAGTTACGTATTCAATATGCGGGTCGCCCGGTTCGTGCGTTCTTCGCGTTTGATCCTGTACGTCAGGCCATT
GTGCTTTGCGCGGGCGATAAGCGTAATGACAAAACCTTTTATGAAAAACTGATTCGCATTGCAGATGCCGAATTCACCCA
TCATCTGACAAGCCTGGAGGCAGCCAAGTGA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 11057.80 Da
• Isoelectric Point: 6.4695

>AT49990 WP_038415012.1 NZ_CP009854:c141519-141220 [Enterobacter cloacae]
VKTLNEVIASRSPESQQRIQEMAEEMFLETGLQLLREELQLSQKHVAALMGVSQPAVTQLEQRGNDLKIATLKRYIEAMG
GKLSINVELPTGKQVAFHL

Nucleotide

• Length: 300 bp

>AT49990 NZ_CP009854:c141519-141220 [Enterobacter cloacae]
GTGAAAACCTTAAATGAAGTGATTGCGTCACGCTCACCGGAAAGCCAACAGCGCATTCAGGAGATGGCAGAGGAAATGTT
CCTCGAAACCGGTCTGCAACTTCTGCGTGAAGAGCTCCAGCTTTCGCAAAAACATGTCGCCGCTCTTATGGGCGTAAGTC
AACCTGCGGTAACACAGCTGGAGCAGCGTGGAAACGATCTGAAGATTGCTACGCTTAAGCGTTACATCGAAGCGATGGGC
GGAAAACTTAGCATTAACGTGGAACTACCCACTGGCAAGCAGGTTGCGTTTCATCTCTAG