Detailed information of TA system TA049862

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/RelE-HTH
• Location: 3457171..3457832
• Replicon: chromosome
• Accession: NZ_CP009801
• Organism: Yersinia intermedia strain Y228

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A0T9MWP7
• Locus tag: CH53_RS15215
• Protein ID: WP_042569386.1
• Coordinates: 3457506..3457832 (-)
• Length: 109 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: CH53_RS15210
• Protein ID: WP_042569385.1
• Coordinates: 3457171..3457467 (-)
• Length: 99 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CH53_RS15190	3452360..3452833	-	474	WP_032905735.1	rRNA maturation RNase YbeY	-
CH53_RS15195	3452830..3453903	-	1074	WP_172668594.1	PhoH family protein	-
CH53_RS15200	3454200..3455624	-	1425	WP_042569384.1	tRNA (N6-isopentenyl adenosine(37)-C2)-methylthiotransferase MiaB	-
CH53_RS15205	3455850..3457031	+	1182	WP_032905763.1	2-octaprenyl-3-methyl-6-methoxy-1,4-benzoquinol hydroxylase	-
CH53_RS15210	3457171..3457467	-	297	WP_042569385.1	helix-turn-helix domain-containing protein	Antitoxin
CH53_RS15215	3457506..3457832	-	327	WP_042569386.1	hypothetical protein	Toxin
CH53_RS15250	3458814..3460478	-	1665	WP_005193277.1	asparagine synthase B	-
CH53_RS15255	3460960..3461712	-	753	WP_032908027.1	HAD-IIA family hydrolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (46-105)

Antitoxin

• Cro/C1-type helix-turn-helix domain (39-82)

Sequences

Toxin

Protein

• Length: 109 a.a.
• Molecular weight: 12525.43 Da
• Isoelectric Point: 9.0960

>T49862 WP_042569386.1 NZ_CP009801:c3457832-3457506 [Yersinia intermedia]
MDFLEFVETPIFSRRRAELMSDDDFQQFQTYLLNNPQSGDTISKTGGCQKIRWGGLQRGKRGGIRVIYFVVAQRCRIWLL
LAYSKNEKDDLSESEKTVLKMITAKLDL

Nucleotide

• Length: 327 bp

>T49862 NZ_CP009801:c3457832-3457506 [Yersinia intermedia]
ATGGATTTCCTTGAGTTTGTAGAAACACCGATTTTTAGTCGGCGTCGAGCAGAACTAATGTCAGATGATGACTTTCAACA
ATTTCAGACTTACCTACTTAATAATCCTCAGTCTGGAGACACAATAAGCAAAACCGGGGGCTGTCAAAAAATTCGATGGG
GTGGTTTGCAGCGAGGTAAACGCGGTGGTATCAGAGTTATCTATTTTGTTGTGGCCCAACGTTGTCGCATCTGGTTATTG
TTGGCTTATTCTAAAAATGAAAAAGATGATCTGTCTGAATCAGAAAAAACCGTATTGAAAATGATAACCGCTAAACTCGA
TTTATAA

Antitoxin

Protein

• Length: 99 a.a.
• Molecular weight: 11268.16 Da
• Isoelectric Point: 7.3203

>AT49862 WP_042569385.1 NZ_CP009801:c3457467-3457171 [Yersinia intermedia]
MKKELFDDLVESMNYMVAAEKGESRPKAEQIHRHPLPDVKLLRLSSGMKQEEFADIVGVSKGLVQSWEQHRRIPSGSALK
LLMMIERNPNIIEELRVL

Nucleotide

• Length: 297 bp

>AT49862 NZ_CP009801:c3457467-3457171 [Yersinia intermedia]
ATGAAAAAAGAACTCTTTGATGATCTGGTTGAAAGCATGAATTACATGGTCGCGGCTGAAAAAGGTGAATCACGCCCTAA
AGCAGAACAAATTCACCGTCACCCCTTACCCGATGTAAAATTATTGCGACTTTCCTCCGGCATGAAACAAGAAGAGTTCG
CAGATATCGTCGGTGTTAGCAAGGGATTGGTTCAAAGCTGGGAGCAACATCGGAGGATCCCTTCAGGTTCAGCATTAAAG
CTATTGATGATGATAGAAAGAAACCCGAATATTATTGAAGAATTACGCGTCCTGTAA

Structures

Toxin

• AlphaFold DB: A0A0T9MWP7