Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 2079357..2079859 Replicon chromosome
Accession NZ_CP009789
Organism Escherichia coli K-12 strain K-12 ER3413

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag ER3413_RS10495 Protein ID WP_000767829.1
Coordinates 2079357..2079611 (-) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag ER3413_RS10500 Protein ID WP_001259255.1
Coordinates 2079608..2079859 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ER3413_RS10470 2074372..2074599 - 228 WP_000234896.1 sulfurtransferase TusA -
ER3413_RS10475 2074613..2075671 - 1059 WP_000492339.1 transport protein YeeE -
ER3413_RS10480 2075850..2077208 - 1359 WP_000019197.1 putrescine/proton symporter PlaP -
ER3413_RS25355 2077198..2077260 - 63 WP_010723108.1 membrane protein YoeI -
ER3413_RS10485 2077475..2078404 - 930 WP_000803351.1 LysR family transcriptional regulator -
ER3413_RS10490 2078450..2079274 - 825 WP_000754737.1 SDR family oxidoreductase -
ER3413_RS10495 2079357..2079611 - 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
ER3413_RS10500 2079608..2079859 - 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
ER3413_RS23680 2080142..2080192 + 51 WP_001364200.1 his operon leader peptide -
ER3413_RS10505 2080338..2081201 + 864 WP_038432600.1 ATP phosphoribosyltransferase -
ER3413_RS10510 2081207..2082511 + 1305 WP_000009594.1 histidinol dehydrogenase -
ER3413_RS10515 2082508..2083578 + 1071 WP_000108941.1 histidinol-phosphate transaminase -
ER3413_RS10520 2083578..2084645 + 1068 WP_000080105.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T49801 WP_000767829.1 NZ_CP009789:c2079611-2079357 [Escherichia coli K-12]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T49801 NZ_CP009789:c2079611-2079357 [Escherichia coli K-12]
GTGAAACTAATCTGGTCTGAGGAATCATGGGACGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT49801 WP_001259255.1 NZ_CP009789:c2079859-2079608 [Escherichia coli K-12]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT49801 NZ_CP009789:c2079859-2079608 [Escherichia coli K-12]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGCTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATCATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References