Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 253341..254035 Replicon chromosome
Accession NZ_CP009789
Organism Escherichia coli K-12 strain K-12 ER3413

Toxin (Protein)


Gene name yafO Uniprot ID Q47157
Locus tag ER3413_RS01210 Protein ID WP_001263489.1
Coordinates 253637..254035 (+) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID S1QAE3
Locus tag ER3413_RS01205 Protein ID WP_000554758.1
Coordinates 253341..253634 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ER3413_RS01185 248973..249470 + 498 WP_000006255.1 REP-associated tyrosine transposase RayT -
ER3413_RS01190 249694..251406 - 1713 Protein_226 flagellar biosynthesis protein FlhA -
ER3413_RS01195 251378..252163 + 786 WP_000207552.1 putative lateral flagellar export/assembly protein LafU -
ER3413_RS01200 252234..253289 + 1056 WP_001226164.1 DNA polymerase IV -
ER3413_RS01205 253341..253634 + 294 WP_000554758.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
ER3413_RS01210 253637..254035 + 399 WP_001263489.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
ER3413_RS01215 254045..254497 + 453 WP_001059892.1 GNAT family N-acetyltransferase -
ER3413_RS01220 254815..255021 + 207 Protein_232 RtcB family protein -
ER3413_RS01225 255017..255538 + 522 Protein_233 peptide chain release factor H -
ER3413_RS01230 255595..257052 - 1458 WP_001292994.1 cytosol nonspecific dipeptidase -
ER3413_RS01235 257313..257771 + 459 WP_001291990.1 xanthine phosphoribosyltransferase -
- 258367..258447 + 81 NuclAT_12 - -
- 258367..258447 + 81 NuclAT_12 - -
- 258367..258447 + 81 NuclAT_12 - -
- 258367..258447 + 81 NuclAT_12 - -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15487.84 Da        Isoelectric Point: 7.4215

>T49782 WP_001263489.1 NZ_CP009789:253637-254035 [Escherichia coli K-12]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANENNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

>T49782 NZ_CP009789:253637-254035 [Escherichia coli K-12]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCCTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGAGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11233.76 Da        Isoelectric Point: 5.2170

>AT49782 WP_000554758.1 NZ_CP009789:253341-253634 [Escherichia coli K-12]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Download         Length: 294 bp

>AT49782 NZ_CP009789:253341-253634 [Escherichia coli K-12]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGCGCT
GAACAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A090J8B1

Antitoxin

Source ID Structure
AlphaFold DB S1QAE3

References