Detailed information of TA system TA049782

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 253341..254035
• Replicon: chromosome
• Accession: NZ_CP009789
• Organism: Escherichia coli K-12 strain K-12 ER3413

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: Q47157
• Locus tag: ER3413_RS01210
• Protein ID: WP_001263489.1
• Coordinates: 253637..254035 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1QAE3
• Locus tag: ER3413_RS01205
• Protein ID: WP_000554758.1
• Coordinates: 253341..253634 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ER3413_RS01185	248973..249470	+	498	WP_000006255.1	REP-associated tyrosine transposase RayT	-
ER3413_RS01190	249694..251406	-	1713	Protein_226	flagellar biosynthesis protein FlhA	-
ER3413_RS01195	251378..252163	+	786	WP_000207552.1	putative lateral flagellar export/assembly protein LafU	-
ER3413_RS01200	252234..253289	+	1056	WP_001226164.1	DNA polymerase IV	-
ER3413_RS01205	253341..253634	+	294	WP_000554758.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
ER3413_RS01210	253637..254035	+	399	WP_001263489.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
ER3413_RS01215	254045..254497	+	453	WP_001059892.1	GNAT family N-acetyltransferase	-
ER3413_RS01220	254815..255021	+	207	Protein_232	RtcB family protein	-
ER3413_RS01225	255017..255538	+	522	Protein_233	peptide chain release factor H	-
ER3413_RS01230	255595..257052	-	1458	WP_001292994.1	cytosol nonspecific dipeptidase	-
ER3413_RS01235	257313..257771	+	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
-	258367..258447	+	81	NuclAT_12	-	-
-	258367..258447	+	81	NuclAT_12	-	-
-	258367..258447	+	81	NuclAT_12	-	-
-	258367..258447	+	81	NuclAT_12	-	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15487.84 Da
• Isoelectric Point: 7.4215

>T49782 WP_001263489.1 NZ_CP009789:253637-254035 [Escherichia coli K-12]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANENNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T49782 NZ_CP009789:253637-254035 [Escherichia coli K-12]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCCTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGAGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.76 Da
• Isoelectric Point: 5.2170

>AT49782 WP_000554758.1 NZ_CP009789:253341-253634 [Escherichia coli K-12]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT49782 NZ_CP009789:253341-253634 [Escherichia coli K-12]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGCGCT
GAACAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Structures

Toxin

• AlphaFold DB: A0A090J8B1

Antitoxin

• AlphaFold DB: S1QAE3