Detailed information of TA system TA049654

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yefM-yoeB (relBE)/YoeB-YefM
• Location: 455472..455974
• Replicon: chromosome
• Accession: NZ_CP009769
• Organism: Pectobacterium brasiliense strain BC1

Toxin (Protein)

• Gene name: yoeB
• Uniprot ID: A0A7W2E5L4
• Locus tag: NC16_RS02075
• Protein ID: WP_039282578.1
• Coordinates: 455472..455726 (-)
• Length: 85 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: -
• Locus tag: NC16_RS02080
• Protein ID: WP_039282574.1
• Coordinates: 455723..455974 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
NC16_RS02055	450608..451870	-	1263	WP_014913996.1	L-rhamnose isomerase	-
NC16_RS02060	451867..453357	-	1491	WP_075277817.1	rhamnulokinase	-
NC16_RS02065	453694..454533	+	840	WP_014913998.1	HTH-type transcriptional activator RhaS	-
NC16_RS02070	454558..455412	+	855	WP_039279287.1	helix-turn-helix domain-containing protein	-
NC16_RS02075	455472..455726	-	255	WP_039282578.1	Txe/YoeB family addiction module toxin	Toxin
NC16_RS02080	455723..455974	-	252	WP_039282574.1	YoeB-YefM toxin-antitoxin system antitoxin YefM	Antitoxin
NC16_RS02085	456050..457759	-	1710	WP_039504943.1	ABC transporter ATP-binding protein	-
NC16_RS02090	457756..458622	-	867	WP_010285293.1	ABC transporter permease	-
NC16_RS02095	458615..459574	-	960	WP_075277818.1	ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (2-84)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-74)

Sequences

Toxin

Protein

• Length: 85 a.a.
• Molecular weight: 10229.79 Da
• Isoelectric Point: 8.0353

>T49654 WP_039282578.1 NZ_CP009769:c455726-455472 [Pectobacterium brasiliense]
VKLIWSEEAWEDYLYWQDIDKRMVKKINELIKETRRTPFEGKGKPEPLKHNLAGFWSRRITEEHRLVYAITDDAMMIAAC
RYHY

Nucleotide

• Length: 255 bp

>T49654 NZ_CP009769:c455726-455472 [Pectobacterium brasiliense]
GTGAAGCTAATCTGGTCAGAAGAAGCATGGGAAGACTACCTGTACTGGCAGGACATCGATAAGCGGATGGTCAAAAAGAT
CAATGAATTAATAAAAGAAACGCGCAGAACACCTTTTGAGGGAAAAGGAAAACCAGAACCACTTAAACATAACCTCGCTG
GTTTCTGGTCGCGCCGTATCACCGAAGAACATCGTCTGGTTTATGCCATCACCGATGACGCCATGATGATCGCTGCCTGC
CGCTACCACTATTAA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9340.61 Da
• Isoelectric Point: 4.6119

>AT49654 WP_039282574.1 NZ_CP009769:c455974-455723 [Pectobacterium brasiliense]
MRTISYSEARQNLSATMMKTVEDRAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANAKRLMSSIESLKAGNGEERD
IIE

Nucleotide

• Length: 252 bp

>AT49654 NZ_CP009769:c455974-455723 [Pectobacterium brasiliense]
ATGCGTACAATTAGCTACAGTGAAGCCCGACAAAATCTATCGGCGACCATGATGAAAACGGTCGAAGACCGTGCTCCCAT
TCTTATCACTCGACAGAATGGTGAAGCCTGCGTGCTCATGTCTTTGGAAGAATATAACTCTCTAGAGGAGACCGCGTATT
TACTGCGTTCACCAGCCAATGCAAAAAGGCTGATGAGCTCAATCGAGAGCCTTAAGGCTGGCAATGGCGAAGAAAGAGAT
ATCATTGAGTGA

Structures

Toxin

• AlphaFold DB: A0A7W2E5L4