Detailed information of TA system TA049645

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/MqsA(antitoxin)
• Location: 85909..86561
• Replicon: plasmid unnamed
• Accession: NZ_CP009763
• Organism: Ralstonia solanacearum OE1-1

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A454TTN3
• Locus tag: RSOE_RS00365
• Protein ID: WP_038962847.1
• Coordinates: 85909..86253 (+)
• Length: 115 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: RSOE_RS00370
• Protein ID: WP_020425351.1
• Coordinates: 86250..86561 (+)
• Length: 104 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
RSOE_RS00350	81604..82767	-	1164	WP_011003438.1	2-methylcitrate synthase	-
RSOE_RS00355	82819..83715	-	897	WP_081365370.1	methylisocitrate lyase	-
RSOE_RS00360	83909..85858	+	1950	WP_023470006.1	propionate catabolism operon regulatory protein PrpR	-
RSOE_RS00365	85909..86253	+	345	WP_038962847.1	hypothetical protein	Toxin
RSOE_RS00370	86250..86561	+	312	WP_020425351.1	transcriptional regulator	Antitoxin
RSOE_RS00375	86701..87510	-	810	WP_043885575.1	DUF2242 domain-containing protein	-
RSOE_RS00380	88755..90593	+	1839	WP_071653875.1	AAA family ATPase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	cyaB / flgC / flgG / fliM / fliG / fliI / acrB / adeG / cheY / cheB / cheW / motA	1..1936579	1936579

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (41-91)

Sequences

Toxin

Protein

• Length: 115 a.a.
• Molecular weight: 13227.94 Da
• Isoelectric Point: 8.4489

>T49645 WP_038962847.1 NZ_CP009763:85909-86253 [Ralstonia solanacearum OE1-1]
MDATFIELPPFQRLREHYLSDDEYRALQQELLARPDAGDVIKGTGGLRKLRFSDKRRGKGKRGGLRVIDYHWDGGGQFWM
FVVYDKGEADDLSSDERKVLARLLEQEVKARSER

Nucleotide

• Length: 345 bp

>T49645 NZ_CP009763:85909-86253 [Ralstonia solanacearum OE1-1]
ATGGATGCTACCTTTATCGAGTTGCCGCCTTTTCAGAGGCTGCGGGAGCACTATCTCTCGGACGACGAGTACCGAGCGCT
CCAACAGGAACTGCTGGCCCGGCCGGACGCCGGCGACGTCATCAAGGGGACAGGTGGCCTACGCAAGCTGCGATTTTCGG
ACAAGCGGCGCGGCAAAGGTAAGCGCGGCGGTCTGCGCGTGATTGACTACCACTGGGATGGAGGCGGTCAATTCTGGATG
TTTGTCGTCTACGACAAGGGCGAGGCGGACGACCTTTCCAGCGACGAGCGCAAAGTGCTGGCACGGCTGTTGGAGCAGGA
AGTCAAGGCAAGGAGTGAGCGATGA

Antitoxin

Protein

• Length: 104 a.a.
• Molecular weight: 11619.65 Da
• Isoelectric Point: 10.7132

>AT49645 WP_020425351.1 NZ_CP009763:86250-86561 [Ralstonia solanacearum OE1-1]
MKRNLFAELKEGMDALAAEREGKMTLRKVKVRVLQPLDVTAEEIKAVRAAAHASQAVMARRLRVNVRTYQNWEQGTAKPN
TQAAVLIKLVEKHPETLQMLEAL

Nucleotide

• Length: 312 bp

>AT49645 NZ_CP009763:86250-86561 [Ralstonia solanacearum OE1-1]
ATGAAACGGAACCTGTTTGCTGAGTTGAAGGAGGGGATGGATGCGCTTGCCGCAGAGCGCGAAGGAAAGATGACCCTTCG
CAAGGTGAAGGTCCGCGTGCTCCAGCCGCTTGACGTGACAGCCGAAGAAATCAAGGCCGTTCGCGCAGCGGCACATGCTT
CACAGGCTGTCATGGCACGTCGACTGCGCGTCAATGTGCGCACCTACCAGAACTGGGAACAAGGAACCGCCAAACCGAAC
ACCCAGGCGGCTGTGTTGATCAAGCTCGTCGAGAAGCATCCGGAAACGTTGCAGATGCTGGAAGCGCTTTAA

Structures

Toxin

• AlphaFold DB: A0A454TTN3