Detailed information of TA system TA049611

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 3115973..3116633
• Replicon: chromosome
• Accession: NZ_CP009756
• Organism: Enterobacter cloacae strain GGT036

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A5E1ABU6
• Locus tag: EC036_RS14755
• Protein ID: WP_020687566.1
• Coordinates: 3116259..3116633 (-)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: EC036_RS14750
• Protein ID: WP_014832568.1
• Coordinates: 3115973..3116275 (-)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EC036_RS14735	3113407..3114366	-	960	WP_038420070.1	zinc-binding alcohol dehydrogenase family protein	-
EC036_RS14740	3114414..3114803	-	390	WP_038420072.1	RidA family protein	-
EC036_RS14745	3114945..3115877	+	933	WP_038420073.1	LysR family transcriptional regulator	-
EC036_RS14750	3115973..3116275	-	303	WP_014832568.1	XRE family transcriptional regulator	Antitoxin
EC036_RS14755	3116259..3116633	-	375	WP_020687566.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
EC036_RS14760	3116992..3117942	-	951	WP_013097829.1	HTH-type transcriptional regulator Cbl	-
EC036_RS14765	3118039..3118956	-	918	WP_010432103.1	nitrogen assimilation transcriptional regulator NAC	-
EC036_RS14775	3119438..3120373	-	936	WP_038420074.1	L,D-transpeptidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	3105797..3116633	10836

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (13-106)

Antitoxin

• Cro/C1-type helix-turn-helix domain (33-79)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 14330.32 Da
• Isoelectric Point: 9.7082

>T49611 WP_020687566.1 NZ_CP009756:c3116633-3116259 [Enterobacter cloacae]
VWTINTTDRFDCWFRSLDDTDRASVLAALLVLREKGPALSRPYADTLKGSRHSNMKELRIQSKGDPLRAFFAFDPNRTGI
VLCAGNKVGNERRFYDEMLLVADREFTHWLNTLKERTLRDGQKP

Nucleotide

• Length: 375 bp

>T49611 NZ_CP009756:c3116633-3116259 [Enterobacter cloacae]
GTGTGGACAATTAACACGACAGACAGGTTCGATTGCTGGTTTCGTTCACTTGACGATACTGATCGGGCCAGCGTGCTGGC
AGCACTGCTTGTTTTGCGAGAAAAGGGGCCTGCTTTGTCGAGGCCCTATGCCGATACGCTTAAAGGCTCTCGTCACAGCA
ACATGAAAGAGCTGCGCATTCAGAGTAAGGGCGATCCGTTGCGGGCATTTTTCGCGTTTGACCCAAACCGTACCGGCATT
GTGCTCTGTGCCGGCAATAAGGTCGGCAACGAAAGACGCTTTTACGATGAAATGCTTCTGGTGGCCGACCGGGAATTCAC
TCACTGGTTGAATACATTAAAAGAGAGGACGTTACGCGATGGGCAGAAACCTTGA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 10972.69 Da
• Isoelectric Point: 5.1473

>AT49611 WP_014832568.1 NZ_CP009756:c3116275-3115973 [Enterobacter cloacae]
MGRNLEQLIADEKSDVVASAQAMAADILLNIHLAELREKVQKTQVEMAQALGIRQPTVAVMEKPGRDLKLSSLKRYVEAA
GGKLTLDVELPDGSHYEFVL

Nucleotide

• Length: 303 bp

>AT49611 NZ_CP009756:c3116275-3115973 [Enterobacter cloacae]
ATGGGCAGAAACCTTGAGCAGCTTATAGCGGATGAAAAATCGGATGTTGTTGCCAGCGCTCAGGCAATGGCTGCCGACAT
TTTGCTTAATATCCATCTGGCAGAGCTACGTGAAAAGGTGCAAAAAACGCAGGTTGAAATGGCGCAGGCGCTGGGCATCC
GACAGCCCACCGTCGCGGTCATGGAAAAGCCCGGGCGCGATCTGAAACTCTCTTCACTCAAGCGCTACGTTGAGGCTGCG
GGTGGAAAGCTTACGCTTGATGTTGAGTTACCGGATGGTTCGCATTATGAGTTTGTGCTGTAG

Structures

Toxin

• AlphaFold DB: A0A5E1ABU6